广西医科大学学报2024，Vol.41Issue(5)：697-707,11.DOI:10.16190/j.cnki.45-1211/r.2024.05.009
富丝氨酸/精氨酸剪接因子7的表达在肝细胞癌中的意义
Significance of expression of serine/arginine-rich splicing factor 7 in hepatocellular carcino-ma
摘要
Abstract
Objective:To investigate the clinical significance and biological functions of serine/arginine-rich splicing factor 7(SRSF7)expression in hepatocellular carcinoma(HCC).Methods:Firstly,the expression levels,clinical pathological features,functions,and pathway enrichment of SRSF7 were analyzed using HCC transcrip-tome and clinical data from TCGA database.Validation was then conducted using clinical HCC samples,Huh7,and Hep3B cell lines,which were divided into control group,overexpression group(SRSF7OE group),and knock-down group(SRSF7KD group).Proliferation and apoptosis abilities were assessed using cell counting kit-8(CCK-8)and TUNEL assays.Migration and invasion abilities were evaluated using wound healing,Transwell migra-tion,and Transwell invasion assays.Epithelial-mesenchymal transition(EMT)-related markers'protein levels were determined by western blotting,while reverse transcription-quantitative PCR(RT-qPCR)was utilized to measure the mRNA levels of downstream regulatory genes of SRSF7.Results:Bioinformatics analysis revealed that SRSF7was highly expressed in HCC tumor tissues and was associated with tumor grade,stage,and poor prognosis(P＜0.05).Western blotting and immunohistochemical results showed that SRSF7 was highly ex-pressed in tumor tissues(P＜0.05).Compared with the control group,overexpression of SRSF7 promoted the pro-liferation,migration,invasion and EMT process of HCC cells;knockdown of SRSF7 could inhibit HCC cell pro-liferation,promote cell apoptosis,and inhibit HCC cell migration,invasion and EMT process(P＜0.05).Conclu-sion:SRSF7 is highly expressed in HCC,and its elevated expression may promote HCC cell proliferation and in-hibit cell apoptosis by regulating the expression of downstream target genes such as PAK5and RTL1,and pro-mote cell migration and invasion by promoting EMT process,ultimately promoting HCC progression.关键词
肝细胞癌/富丝氨酸/精氨酸剪接因子7/增殖/迁移侵袭/上皮—间质转化Key words
hepatocellular carcinoma/serine/arginine-rich splicing factor 7/proliferation/migration and inva-sion/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
唐文,张飞云,韦彩妮,赵玉玲,刘耀鸿,黎东心,黄秒,胡启平..富丝氨酸/精氨酸剪接因子7的表达在肝细胞癌中的意义[J].广西医科大学学报,2024,41(5):697-707,11.基金项目
国家自然科学基金资助项目(No.81760424) （No.81760424）
广西自然科学基金资助项目(2024GXNSFAA010034) （2024GXNSFAA010034）
广西医科大学2022年大学生创业训练计划立项项目资助(No.S202210598062) （No.S202210598062）
