失眠颗粒对疫情后PTSD患者卒中预防作用的网络药理学及动物实验研究OA北大核心CSTPCD

Objective:The mechanism of treating post-traumatic stress disorder(PTSD)with insomnia granules in Shaanxi Provincial Hospital of Traditional Chinese Medicine in the long term prevention of stroke was investigated by network pharmacolo-gy and animal experiments.Method:TCMIP and BATMAN-TCMTCMSP databases were used to screen the effective compo-nents and targets of various Chinese herbs in the composition of insomnia particles.The differential genes of PTSD and stroke were obtained by gene coexpression network analysis(WGCNA)based on GEO database and gene expression mapping analysis technology.Data mining and statistical analysis were performed using R language software to screen for risk genes for stroke in PTSD.The target of insomnia particles was intersected with the risk gene of stroke in PTSD.The target interaction network was constructed by using STRING data library.Topological analysis was carried out on Cytoscape software to screen core targets,and GO function analysis and KEGG concentration analysis were carried out on the intersection targets.The core target was selected for animal experiments.Result:The insomnia granules were screened for 642 compound components corresponding to 2 082 tar-gets,and 2 379 risk genes for stroke in PTSD were screened.Totally 165 common targets of insomnia granules components and diseases were obtained by VENN analysis.The PPI analysis sorted out 68 key targets,which are the core targets of insomnia gran-ules for the treatment of PTSD and for the long-term prevention of stroke.Topology analysis was performed at CytoNCA and the top 10 core targets were MAPK3,MAPK1,NCOA2,MAPK14,NR3C1,RARA,PRKACA,SMARCD3,FOS,andFOXO1.KEGG enrichment analysis yielded 104 related pathways,and GO functional analysis yielded 10 BP pathways,10 MF pathways,and 3 CC pathways.In animal experiments,compared with the model group,insomnia granules significantly im-proved anxiety-like behavior in PTSD,and simultaneously reduced the expression level of p38 MAPK protein.Conclusion:In-somnia granules inhibit depressive-anxiety-like behaviors and memory capacity in post-traumatic stress disorder by inhibiting the p38 MAPK-mediated MAPK pathway.The incidence of stroke among patients with PTSD is high,and the effectiveness of insom-nia pellets in treating PTSD and for long-term prevention of stroke is documented.