广西医科大学学报2024，Vol.41Issue(5)：722-727,6.DOI:10.16190/j.cnki.45-1211/r.2024.05.012
上调miR-21-5p对阿霉素诱导的心肌细胞增殖、凋亡的影响及其机制研究
Effect and mechanism of up-regulation of miR-21-5p on doxorubicin-induced cardiomyo-cyte proliferation and apoptosis
摘要
Abstract
Objective:To investigate the effect and mechanism of up-regulation of miR-21-5p on doxorubicin(DOX)-induced cardiomyocyte proliferation and apoptosis.Methods:Cardiomyocytes H9C2 were divided into control group(conventional-cultured control cells),DOX group(cultured with 1 μM DOX),NC agomir group(DOX+NC ago group),miR-21-5p agomir group(DOX+miR-21-5p ago group),NC antagomir treatment group(DOX+NC anta group),and miR-21-5p antagomir treatment group(DOX+miR-21-5p anta group).The expres-sion of miR-21-5p was detected by reverse transcription-quantitative polymerase chain reaction(RT-qPCR),cell proliferation was detected by MTT,cell apoptosis was detected by flow cytometry,and the protein expression of C-Caspase-3,β-catenin and c-Myc was detected by western blotting.Results:Compared with the control group,the level of miR-21-5p and cell proliferation activity were decreased,apoptosis and C-Caspase-3 protein expres-sion levels were increased,and β-catenin and c-Myc protein expression levels were decreased in the DOX group(P＜0.05).Compared with the DOX+NC ago group,the level of miR-21-5p in the DOX+miR-21-5p ago group was increased,cell proliferation activity was increased,apoptosis and C-Caspase-3 protein expression levels were decreased,and β-catenin and c-Myc protein expression levels were increased(P＜0.05).Conclusion:Up-regula-tion of miR-21-5p promotes DOX-induced cardiomyocyte proliferation and inhibits DOX-induced cardiomyo-cyte apoptosis through Wnt/β-catenin signaling pathway,thereby ameliorating DOX-induced cardiotoxicity.关键词
心肌细胞/miR-21-5p/Wnt/β-catenin/凋亡/阿霉素Key words
cardiomyocyte/miR-21-5p/Wnt/β-catenin/apoptosis/doxorubicin分类
医药卫生引用本文复制引用
耿洁,刘洁婕,刘亚,贺赛..上调miR-21-5p对阿霉素诱导的心肌细胞增殖、凋亡的影响及其机制研究[J].广西医科大学学报,2024,41(5):722-727,6.基金项目
陕西省重点研发计划资助项目(No.2021SF-218) （No.2021SF-218）
陕西省自然科学基础研究计划资助项目(No.2018JQ8044) （No.2018JQ8044）
