广西医科大学学报2024，Vol.41Issue(5)：728-733,6.DOI:10.16190/j.cnki.45-1211/r.2024.05.013
基于氧化应激介导NF-κB通路研究ALDH2基因表达对氯胺酮相关性膀胱炎的影响
Effect of ALDH2 gene expression on ketamine-induced cystitis based on oxidative stress-me-diated NF-κB pathway
摘要
Abstract
Objective:To investigate the effect of acetaldehyde dehydrogenase 2(ALDH2)gene expression on oxidative stress-mediated NF-κB pathway in ketamine-induced cystitis(KIC).Methods:SV-HUC-1 cells were divided into different concentrations of ketamine groups(0 mmol/L,0.5 mmol/L,1 mmol/L,2 mmol/L),and the expression of ALDH2 protein was detected after induction for 48 hours.The SV-HUC-1 cell constructs were di-vided into control group(si-NC group,OE-NC group),ALDH2 silencing group(Si-ALDH2 group),and ALDH2 overexpression group(OE-ALDH2 group).The cells were induced with complete medium and ketamine(1 mmol/L)for 48 hours respectively,and the levels of inflammatory factors and inflammatory proteins were detected in each group.Results:Western blotting showed that the relative expression of ALDH2 protein after treatment with ketamine(0.5 mmol/L,1 mmol/L)was significantly higher than that in the 0 mmol/L group(P＜0.05).In the fur-ther comparison of ketamine-induced group(1 mmol/L),it was found that the expression of inflammatory cyto-kines interleukin-1β(IL-1β)and interleukin-6(IL-6)in the si-ALDH2 group was significantly higher than that in the si-ALDH2 group(P＜0.05);the expression of IL-1β and IL-6 in the OE-ALDH2 group was significantly low-er than that in the OE-NC group(P＜0.05).The expression levels of inflammatory protein nuclear factor-κB(NF-κB),cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS)in the si-ALDH2 group were higher than those in the si-NC group(P＜0.05).The expression levels of inflammatory proteins NF-κB,COX-2 and iN-OS in the OE-ALDH2 group were lower than those in the OE-NC group(P＜0.05).In the control group(0 mmol/L),there were no significant differences in the levels of inflammatory factors and inflammatory proteins between si-ALDH2 group and si-NC group,as well as OE-ALDH2 group and OE-NC group(P＞0.05).Conclusion:AL-DH2gene may play a certain anti-inflammatory protective role in the occurrence of KIC.关键词
氯胺酮相关性膀胱炎/乙醛脱氢酶/氧化应激Key words
ketamine-induced cystitis/acetaldehyde dehydrogenase 2/oxidative stress分类
医药卫生引用本文复制引用
章民昊,奚晓剑,顾鹏,何晓亮..基于氧化应激介导NF-κB通路研究ALDH2基因表达对氯胺酮相关性膀胱炎的影响[J].广西医科大学学报,2024,41(5):728-733,6.基金项目
江苏省无锡市卫生健康委科研青年项目(No.Q202030) （No.Q202030）
