河南农业科学2024，Vol.53Issue(6)：91-99,9.DOI:10.15933/j.cnki.1004-3268.2024.06.010
黄色小长蝽的线粒体基因组测序和分析
The Mitochondrial Genome Sequencing and Analysis of Nysius inconspicuus
摘要
Abstract
To explore the mitochondrial genome structure of Nysius inconspicuus and the phylogeny of Lygaeidae,we newly sequenced the complete mitochondrial genome of N.inconspicuus using next-generation sequencing methods.In phylogenetic analyses,we selected 14 representative species of Lygaeidae as ingroups.One species of Geocoridae was used as outgroup.We reconstructed the phylogenetic relationships using Maximum likelihood and Bayesian inference methods.The results indicated that the mitochondrial genome of N.inconspicuus encompassed 37 genes(13 protein-coding genes,22 transfer RNA genes,and 2 ribosomal RNA genes)along with 1 non-coding control region,spanning a total length of 14 893 bp(GenBank accession number:OR099699).In the mitochondrial genome of N.inconspicuus,the start codons of protein-coding genes were TTG for cox1 and nad6,AAC for nad4l,while the remaining protein-coding genes began with ATN.As for the stop codons,cox1,cox2,nad3,and nad1 ended with an incomplete T,whereas the rest of protein-coding genes terminated with TAA.In addition,based on three different data matrices,we employed two phylogenetic analysis methods,resulting in largely consistent phylogenetic trees.关键词
黄色小长蝽/半翅目/长蝽科/线粒体基因组/系统发育Key words
Nysius inconspicuus/Hemiptera/Lygaeidae/Mitochondrial genome/Phylogeny分类
农业科技引用本文复制引用
林兴雨,席玉强,宋南,尹新明..黄色小长蝽的线粒体基因组测序和分析[J].河南农业科学,2024,53(6):91-99,9.基金项目
国家自然科学基金项目(U1904104) （U1904104）
国家现代农业体系研究项目(CARS-27) （CARS-27）
