12C6+辐射对菊苣根黄酮类物质及抗氧化活性的影响OA北大核心CSTPCD

To investigate the effects of 12C6+radiation on flavonoids and antioxidant activity in chicory,chicory roots were used as experimental materials to determine the total flavonoid content and antioxidant activity under different 12C6+radiation doses.Ultra-high performance liquid chromatography-electrospray ionization tandem four-stage rod mass spectrometry(UPLC-ESI-MS/MS)was used to conduct targeted metabonomic analysis of flavonoids in 20 and 40 Gy radiation groups which had significantly increased total flavonoids content and control groups.The results showed that 10-40 Gy 12C6+radiation increased the total flavonoid content of chicory roots,with the most significant increase in the 20 and 40 Gy radiation groups.Five categories of 11 flavonoid metabolites were identified,with hesperetin and cyanidin 3-O-rutinoside chloride being the key differential metabolites in response to the increase in total flavonoid content of chicory roots caused by 12C6+radiation.12C6+radiation affected the synthesis of astragalin,isorhamnetin-3-O-glucoside,luteolin,and hesperetin.Flavonoid biosynthesis pathway(ko00941)and Flavone and flavonol biosynthesis pathway(ko00944)were significantly enriched.20 and 40 Gy radiation increased the DPPH,ABTS+radical scavenging ability,and ferric ion reduction ability(FRAP)of flavonoid extract from chicory root,and significantly enhanced its antioxidant activity.In summary,12C6+radiation at doses of 20 and 40 Gy can increase the total flavonoid content,alter the types and content of flavonoids in chicory roots,and enhance their antioxidant activity.The results of this study provide a theoretical basis for further research on radiation induced breeding of chicory.