河南科技大学学报（自然科学版）2024，Vol.45Issue(4)：73-78,6.DOI:10.15926/j.cnki.issn1672-6871.2024.04.010
奶牛小肠上皮细胞系细胞染色体核型与G-带分析
Chromosome Karyotype and G-band Analysis of Bovine Small Intestinal Epithelial Cell Line
摘要
Abstract
In order to identify the genetic stability in biology of bovine small intestinal epithelial cell line(BIECs-21),BIECs-21 cells were cultured in vitro by using adherent culture,and chromosome and G-band samples were prepared to analyze the chromosome karyotype and G-band of BIECs-21 cells.The results show that the number of chromosomes in BIECs-21 cells is 2n=60 including 29 pairs of autosomes and 1 pair of sex chromosomes(X and Y chromosomes).Among them,29 pairs of autosomes are all terminal centromere chromosomes,with X chromosome being the larger centromere chromosome and Y chromosome being the smaller centromere chromosome in the sex chromosome.There is no significant difference in the number and position of G-bands pattern on the autosomes of BIECs-21 cells,except for X and Y sex chromosome.Compared with the normal chromosome karyotype of Holstein cattle,there is no significant change in the number and morphological structure of chromosomes in BIECs-21 cells,indicating that the genetic characteristics of the cell line are stable,and it can be used for the reaearch on the molecular mechanisms of bovine intestinal related diseases.关键词
BIECs-21细胞/贴壁培养/染色体核型分析/G-带分析Key words
BIECs-21 cell/adherent culture/chromosome karyotype analysis/G-band analysis分类
生物科学引用本文复制引用
王雪莹,孟素丹,何雷,赵淑娟,王聪慧,李日顺,钱伟锋,张才..奶牛小肠上皮细胞系细胞染色体核型与G-带分析[J].河南科技大学学报（自然科学版）,2024,45(4):73-78,6.基金项目
国家自然科学基金项目(31872537) （31872537）
国家重点研发计划项目(2022YFE0111100) （2022YFE0111100）
