昆明医科大学学报2024，Vol.45Issue(6)：15-21,7.DOI:10.12259/j.issn.2095-610X.S20240603
TRPC1与BK-α的表达对大鼠糖尿病肾病的影响
Exploring the Effect of TRPC1 and BK-α expression on Diabetic Nephropathy
摘要
Abstract
Objective To explore the effect of transient receptor potential channel 1(TRPC1)protein and large conductivity Ca2+activated K+channel α subunit(BK-α)protein on diabetic nephropathy(DKD).Methods SD rats were randomly divided into a control group(n=15)and a model group(n=15).Construct a DKD model using high-fat feed and streptozocin(STZ).Using a blood glucose analyzer to detect changes in blood glucose levels in rats;Using a fully automatic biochemical analyzer to detect the renal function level of rats;HE staining was used to detect pathological changes in renal tissue to confirm successful modeling.Real time fluorescence quantitative PCR(RT-qPCR)and Western blotting were used to detect the mRNA and protein expression levels of TRPC1 and BK-α in renal tissue,respectively;Immunohistochemical detection of the distribution and expression of TRPC1 and BK-α.Results The fasting plasma glucose(FPG),urinary albumin excretion rate(UAER),blood urea nitrogen(BUN),and creatinine(Cr)in the model group were significantly higher than those in the control group(P<0.01);the inner wall cells of the renal tubules in the model group showed swelling,with some cells detached;visible renal tubular lesions or death.In addition,neutrophils and their debris were found in many renal tubules and interstitial regions;the above HE staining results indicate that the DKD model has been successfully replicated.TRPC1 and BK-α were most abundant in the glomerulus,and the mRNA and protein levels of TRPC1 and BK-α in the renal tissue of the model group rats were significantly higher than those in the control group(P<0.05).Conclusion Dia-betic nephropathy affects the distribution and expression of TRPC1 and BK-α in renal tissue.关键词
大鼠糖尿病肾病/瞬时受体电位C1蛋白/大电导钙离子激活钾通道α亚单位蛋白Key words
Diabetic nephropathy/Transient receptor potential channel 1/Large conductance Ca2+-activated K+channel α subunit分类
医药卫生引用本文复制引用
刘红明,陈志松,邹立芳,杨智雄,喻卓,胡伟..TRPC1与BK-α的表达对大鼠糖尿病肾病的影响[J].昆明医科大学学报,2024,45(6):15-21,7.基金项目
云南省科技厅基础研究计划基金资助项目(202001AY070001-184) （202001AY070001-184）
云南省心脑血管临床医学研究中心子课题基金资助项目(ZX2019-03-01) （ZX2019-03-01）
云南省放射与治疗临床医学研究中心子课题基金资助项目(202102AA100067). （202102AA100067）
