昆明医科大学学报2024，Vol.45Issue(6)：36-43,8.DOI:10.12259/j.issn.2095-610X.S20240605
袖状胃切除术对肥胖小鼠糖代谢及自噬活性的影响
Effect of Sleeve Gastrectomy on Glucose Metabolism and Autophagy in Obese Mice
摘要
Abstract
Objective To investigate the changes in liver autophagy factor activity in obese mice before and after sleeve gastrectomy(SG).Methods Male obese mice aged 6-8 weeks were randomly divided into a sham surgery group(Sham group,n=6)and a sleeve gastrectomy surgery group(SG group,n=6).The daily weight and food intake of the two groups of mice were recorded within one month after surgery,and the fasting blood glucose values at the tip of the mouse tail were measured at 15 and 30 days after surgery.Oral glucose tolerance tests were performed to evaluate glucose absorption;RT-qPCR was used to detect the mRNA expression changes of autophagy factors and glucose transporters at 15 and 30 days after surgery.Results Compared with preoperation,there was no statistically significant difference(P>0.05)in the weight,daily food intake,and fasting blood glucose values of the sham surgery group at 15 and 30 days after surgery,while the SG group had a decrease in weight,daily food intake,and fasting blood glucose values at 15 and 30 days after surgery and had a statistically significant difference(P<0.05);Compared with the sham surgery group,the weight,daily food intake,and fasting blood glucose of the SG group decreased and showed statistical differences(P<0.05)at 15 and 30 days.The autophagy factor P62 in the SG group mice increased,while the mRNA expression of Beclin-1,LC3I/LC3II,and SGLT1 decreased and showed statistical differences(P<0.05)at 15 and 30 days after surgery.Conclusion SG can improve obesity and reduce inflammatory mediators and adipokines,further regulating autophagic activity in mouse liver.关键词
袖状胃切除术/肥胖小鼠糖代谢/自噬活性Key words
Sleeve-shaped gastrectomy/Glucose metabolism in obese mice/Autophagy activity分类
医药卫生引用本文复制引用
夏军,李伟思,张文杰,戚宗泽..袖状胃切除术对肥胖小鼠糖代谢及自噬活性的影响[J].昆明医科大学学报,2024,45(6):36-43,8.基金项目
昆明市卫生科技基金资助项目(202104010010) （202104010010）
昆明医科大学研究生教育创新基金资助项目(2022S288) （2022S288）
