昆明医科大学学报2024，Vol.45Issue(6)：44-50,7.DOI:10.12259/j.issn.2095-610X.S20240606
油性坚果摄入量与原发性肺癌的两样本孟德尔随机化研究
A Two-sample Mendelian Randomization Study of Oily Nut Intake and Primary Lung Cancer
摘要
Abstract
Objective To investigate the potential causal relationship between oily nut consumption and primary lung cancer by two-sample Mendelian randomization method.Methods Single nucleotide polymorp hisms(P<5×10-7)associated with oily nut intake were screened from the openGWAS public database of genome-wide association study as instrumental variables.The inverse variance weighting method was the main analysis method to evaluate the causal effect of this study,and MR-Egger regression and weighted median method were used to supplement the explanation.OR value and 95%CI were used to evaluate whether there was a causal relationship between oily nut intake and primary lung cancer.The mr_pleiotropy_test and MR-PRESSO were used to test the level pleiotropy,and the leave-one-out method was used to test the sensitivity.Results A total of 100 SNPs were extracted from the ukb-b-16576 dataset as instrumental variables.The MR-Egger regression,weighted median,and inverse variance weighting methods yielded OR values and 95%confidence intervals[0.63,95%CI(0.11-3.65),P=0.611],[0.34,95%CI(0.19-0.61),P=0.001],[0.32,95%CI(0.21-0.48),P=0.001],rerespectively.The mr_pleiotropy_test showed that the MR-Egger intercept was close to 0(-7.66E-03)with a p-value of 0.441,indicating no significant horizontal pleiotropy.Similarly,the MR-PRESSO package GlobalTest yielded a p-value of 0.162,further confirming the absence of horizontal pleiotropy among the selected instrumental variables.After conducting the leave-one-out sensitivity analysis,no significant influence on the results was observed from any specific SNP locus,indicating that the results are highly stable and reliable.Conclusions The two-sample Mendelian randomization analysis revealed a negative causal relationship between oily nut intake and the risk of primary lung cancer.However,further verification is needed with larger sample sizes and data from individuals of different ethnic backgrounds to strengthen the findings.关键词
油性坚果/原发性肺癌/孟德尔随机化/因果推断Key words
Oily nuts/Primary lung cancer/Mendel randomization/Causal inference分类
医药卫生引用本文复制引用
郭学云,李娜,陶国仲,黄正..油性坚果摄入量与原发性肺癌的两样本孟德尔随机化研究[J].昆明医科大学学报,2024,45(6):44-50,7.基金项目
中央级公益性科研院所基本科研业务费专项资金资助项目(CAFYBB20ZI001-04) （CAFYBB20ZI001-04）
滁州市卫生健康科研课题基金资助项目(CZWJ2022C004) （CZWJ2022C004）
