内科理论与实践2024，Vol.19Issue(2)：115-120,6.DOI:10.16138/j.1673-6087.2024.02.05
匹诺塞林通过抑制自噬减轻慢性间歇性缺氧诱导的BEAS-2B细胞损伤
Pinocembrin alleviates BEAS-2B cell damage induced by chronic intermittent hypoxia through inhibiting autophagy
摘要
Abstract
Objective To investigate the effect of pinocembrin(PIN)on BEAS-2B human bronchial epithelial cell injury induced by chronic intermittent hypoxia(CIH)and its possible regulatory mechanism.Methods The optimal CIH intervention duration and PIN treatment concentration were determined by cell counting kit 8(CCK8)in BEAS-2B cells.BEAS-2B cells were divided into control group(CON group),CIH group and CIH+PIN group.The proliferation activity of BEAS-2B cells in different treatment groups was detected by CCK8.Western blotting(WB)was used to detect the protein expression levels of hypoxia-inducible factor 1α(HIF-1α),apoptosis related gene[cleaved caspase 3,B cell lymphoma 2(Bcl-2),Bcl-2 associated X protein(Bax)]and autophagy related genes[light chain 3(LC3)-Ⅱ,beclin-1,P62].After adding the autophagy inhibitor 3-methyladenine(3-MA),the cells were divided into five groups:CON group,CIH group,CIH+3-MA group,CIH+PIN group,and CIH+3-MA+PIN group,and the expression levels of HIF-1α,apoptosis and autophagy related genes in each group were detected.Results CCK8 showed that the proliferation activity of BEAS-2B cells significantly decreased between 24-48 h with the prolongation of hypoxia time.PIN could up-regulate the proliferative activity of BEAS-2B cells under CIH.The results of WB showed that compared with CON group,the protein expressions of HIF-1α,Bax,LC3-Ⅱ and beclin-1 were increased in CIH group(P<0.05).The expression of P62 and anti-apoptosis gene Bcl-2 protein was significantly down-regulated(P<0.05).After adding 3-MA,the protein expression level of HIF-1α was reduced,autophagy and apoptosis were inhibited.The same trend was observed after adding PIN.Conclusions PIN can reduce CIH-induced BEAS-2B cell damage and exert a protective effect.The mechanism may be related to down-regulating autophagy level and inhibiting cell apoptosis.关键词
匹诺塞林/慢性间歇性缺氧/自噬/凋亡Key words
Pinocembrin/Chronic intermittent hypoxia/Autophagy/Apoptosis分类
医药卫生引用本文复制引用
何嫣婕,何美娟,王韵,朱春雪,黄汉鹏..匹诺塞林通过抑制自噬减轻慢性间歇性缺氧诱导的BEAS-2B细胞损伤[J].内科理论与实践,2024,19(2):115-120,6.基金项目
江苏省科技项目(BE2021694) （BE2021694）
镇江"金山英才"高层次领军人才培养计划项目(YLJ202105) （YLJ202105）
