胰岛素淀粉样纤维的近红外聚集诱导发光荧光探针OACSTPCD
Aggregation-induced emission(AIE)active NIR probe of insulin amyloid fibrils
为了快速检测淀粉样纤维以及深入研究淀粉样纤维化过程的动力学和结构特性的机理,实现对阿尔茨海默病(AD)等的早期监测,本文设计了一种两亲性的聚集诱导发光(AIE)荧光探针(E)-1-丁基-4-(4-(二苯胺基)苯乙烯基)吡啶-1-溴化鎓(BSTI).荧光探针BSTI表现出高的结合亲和力、高的开关比、高的光稳定性和良好的双光子激发荧光特性,并兼具适当的相对分子质量以及可穿越血脑屏障的潜能.当其与牛胰岛素淀粉样原纤维结合后,表现出强烈的近红外(NIR)荧光发射,开关比＞100.此外,基于BSTI的淀粉样原纤维的双光子荧光成像也表现出较高的信噪比.BSTI有望成为检测体内淀粉样原纤维的理想探针,并应用于AD的早期监测.
In order to study the mechanism of rapid detection of amyloid fibrils and the kinetic and structural properties of the amyloid fibrillation process for early monitoring of Alzheimer'disease(AD),this paper designed an amphiphilic AIE fluorescent probe(E)-1-butyl-4-(4-(diphenylamino)styryl)pyridin-1-ium bromide(BSTI).The BSTI exhibits high binding affinity,high on-off ratio,high photostability and good two-photon excitation fluorescence properties,as well as appropriate molecular weight and the potential to cross the blood-brain barrier.When combined with bovine insulin amyloid fibrils,it exhibits strong NIR emission with an on/off ratio＞100.In addition,BSTI-based two-photon fluorescence imaging of amyloid fibrils also exhibited a high signal-to-noise ratio.Which make BSTI an ideal probe for detecting amyloid fibrils in vivo and early monitoring of AD.
常子彦;姚颖芳;徐珍珍;廖清;付红兵
首都师范大学化学系,光功能材料与器件重点实验室,北京 100048
化学
聚集诱导发光荧光探针胰岛素淀粉样原纤维近红外发射
aggregation-induced luminescencefluorescent probeinsulin amyloid fibrilsnear-infrared emission
《首都师范大学学报（自然科学版）》 2024 (003)
15-24 / 10
国家自然科学基金项目(22275125,22150005,22090022);国家重点研发计划项目(2022YFA1204402,2018YFA0704805,2018YFA0704802);北京市自然科学基金项目(KZ202110028043);北京市教育委员会研发计划项目(KM202210028016,BPHR202203119)
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