首都师范大学学报（自然科学版）2024，Vol.45Issue(3)：15-24,10.DOI:10.19789/j.1004-9398.2024.03.002
胰岛素淀粉样纤维的近红外聚集诱导发光荧光探针
Aggregation-induced emission(AIE)active NIR probe of insulin amyloid fibrils
摘要
Abstract
In order to study the mechanism of rapid detection of amyloid fibrils and the kinetic and structural properties of the amyloid fibrillation process for early monitoring of Alzheimer'disease(AD),this paper designed an amphiphilic AIE fluorescent probe(E)-1-butyl-4-(4-(diphenylamino)styryl)pyridin-1-ium bromide(BSTI).The BSTI exhibits high binding affinity,high on-off ratio,high photostability and good two-photon excitation fluorescence properties,as well as appropriate molecular weight and the potential to cross the blood-brain barrier.When combined with bovine insulin amyloid fibrils,it exhibits strong NIR emission with an on/off ratio＞100.In addition,BSTI-based two-photon fluorescence imaging of amyloid fibrils also exhibited a high signal-to-noise ratio.Which make BSTI an ideal probe for detecting amyloid fibrils in vivo and early monitoring of AD.关键词
聚集诱导发光/荧光探针/胰岛素淀粉样原纤维/近红外发射Key words
aggregation-induced luminescence/fluorescent probe/insulin amyloid fibrils/near-infrared emission分类
化学化工引用本文复制引用
常子彦,姚颖芳,徐珍珍,廖清,付红兵..胰岛素淀粉样纤维的近红外聚集诱导发光荧光探针[J].首都师范大学学报（自然科学版）,2024,45(3):15-24,10.基金项目
国家自然科学基金项目(22275125,22150005,22090022) （22275125,22150005,22090022）
国家重点研发计划项目(2022YFA1204402,2018YFA0704805,2018YFA0704802) （2022YFA1204402,2018YFA0704805,2018YFA0704802）
北京市自然科学基金项目(KZ202110028043) （KZ202110028043）
北京市教育委员会研发计划项目(KM202210028016,BPHR202203119) （KM202210028016,BPHR202203119）
