XRN4/EIN5调控拟南芥对盐胁迫和ABA的耐受性OA北大核心CSTPCD

XRN4/EIN5 is a 5'-3'exonuclease in plants,which is mainly involved in degradation of RNA in cytoplasm,and also is a core gene of ethylene signaling pathway.Therefore,it plays important roles in plant growth and development and ethylene signaling transduction.In order to explore its function on stress resist-ance,the XRN4/EIN5 T-DNA insertion mutant ein5-6 was used as material,and different concentrations of NaCl(0,50,100,150,200 mmol/L)and ABA(0,0.10,0.25,0.50,1.00,1.50 μmol/L)were set for the treatments.Based on the results of seed germination rate,cotyledon greening rate,leaf number and leaf ar-ea,it was found that when the salt concentration was lower than 50 mmol/L,the germination rate,cotyledon greening rate,leaf number and leaf area of ein5-6 were higher than those of the wild-type Col-0;when the salt concentration reached 100 mmol/L,the germination rate and cotyledon greening rate of ein5-6 were higher at early stage of growth but lower after treated for 60 h and 96 h compared with Col-0,and there was no differ-ence in leaf number and leaf area between them.When the salt concentration reached 150 mmol/L and above,the germination rate,cotyledon greening rate,leaf number and leaf area of ein5-6 were lower than those of Col-0.In addition,the response of ein5-6 to ABA was consistent with that to salt stress.When ABA concentra-tion was low,the cotyledon greening rate of ein5-6 was higher than that of Col-0,but that decreased with the extension of treating time and the increase of ABA concentration,and finally obviously lower than that of Col-0.These results indicated that ein5-6 was more sensitive to the salt stress and ABA treatment,so it was speculated that XRN4/EIN5 be involved in the regulation of salt stress and ABA stress responses.