山东农业科学2024，Vol.56Issue(6)：55-64,10.DOI:10.14083/j.issn.1001-4942.2024.06.008
基于品质性状与矿质元素含量的多花黄精种源多样性分析及评价
Provenance Diversity Analysis and Evaluation of Polygonatum cyrtonema Hua Based on Quality Traits and Mineral Element Content
摘要
Abstract
In order to study the genetic diversity of quality traits and mineral elements of Polygonatum cyrtonema Hua germplasm resources and screen high-quality germplasms,43 P.cyrtonema germplasm re-sources in China were collected,and the contents of 7 quality traits and 15 mineral elements were determined,and a comprehensive evaluation was conducted by diversity analysis,correlation analysis,principal component analysis,clustering analysis and membership function method.The results showed that the contents of polysac-charide,total ash,alcohol-soluble extract,water,and some heavy metals and harmful elements in the germ-plasm resources met the standards of China Pharmacopoeia(2020 edition).The variation coefficient of quality traits was 10.4%to 46.7%,and the genetic diversity index was 1.600 to 2.015.The variation coefficient of mineral elements was 23.6%to 92.7%,and the genetic diversity index was 1.526 to 2.006.Correlation analy-sis results showed that significant(P<0.05)or extremely significant(P<0.01)levels were reached in a total of 76 pairs of indicators.The correlation between mineral elements was complex but the overall synergistic effect was strong,and the inhibition effect of mineral elements on quality traits was stronger.Eleven mineral elements were simplified into three principal components by principal component analysis with the comulative contribution rate as 66.71%,and K,Zn,Fe and P were screened out as the characteristic elements for evalu-ating the germplasm resources of P.cyrtonema.The 43 germplasm resources were divided into four groups at the distance of 100 when analyzed by systematic clustering,and the result was basically consistent with that of comprehensive evaluation by membership function method,and the top ten high-quality provenances were S33,S27,S29,S28,S31,S10,S17,S36,S32,and S20,respectively.In conclusion,there were obviously variation in quality traits and mineral elements among the germplasm resources of P.cyrtonema,which showed rich genetic diversity.The results could provide a reference for breeding high quality P.cyrtonema.关键词
多花黄精/种质资源/品质性状/矿质元素/遗传多样性/综合评价Key words
Polygonatum cyrtonema Hua/Germplasm resources/Quality traits/Mineral elements/Ge-netic diversity/Comprehensive evaluation分类
农业科技引用本文复制引用
王灿彬,罗夫来,黄明进,潘克琴,王华磊,罗春丽,李金玲,陈松树,李丹丹,梁瑾,刘红昌..基于品质性状与矿质元素含量的多花黄精种源多样性分析及评价[J].山东农业科学,2024,56(6):55-64,10.基金项目
国家重点研发计划项目"林下生态种植黄精、淫羊藿等中药材产业关键技术研究与应用示范"(2021YFD1601002) （2021YFD1601002）
黄精种质资源收集保存鉴定评价(黔农计财[2022]10号) （黔农计财[2022]10号）
贵州省药用植物繁育与种植人才基地项目(黔人领发[2020]2号) （黔人领发[2020]2号）
贵州特色药用植物种苗繁育工程技术研究中心项目(黔科合平台人才[2020]2101号) （黔科合平台人才[2020]2101号）
基于DNA分子标记的黄精遗传多样性研究及优异种质资源筛选(筑科合同[2022]3-3号) （筑科合同[2022]3-3号）
贵州省中药材现代农业产业技术体系项目(GZCYTX2024) （GZCYTX2024）
道地药材黄精高端产品研发及产业化应用研究(铜科成合[2020]02号) （铜科成合[2020]02号）
