山东农业科学2024，Vol.56Issue(6)：81-87,7.DOI:10.14083/j.issn.1001-4942.2024.06.011
枯草芽孢杆菌对土壤真菌群落结构及草莓根腐病的调控作用
Regulation of Bacillus subtilis on Soil Fungal Community Structure and Root Rot of Strawberry
摘要
Abstract
In order to explore the regulation of Bacillus subtilis on soil fungal community structure and crop growth,high-throughput sequencing technology was used to analyze the effects of B.subtilis on fungal di-versity and community structure in strawberry rhizosphere soil,and the effects on controlling strawberry root rot and promoting strawberry growth were determined by field plot experiment.The results showed that the applica-tion of B.subtilis significantly increased the fungal diversity in strawberry rhizosphere soil.The Shannon index,Ace index and Chao index increased significantly,and the Simpson index decreased significantly.At phylum level,the relative abundance of Ascomycota increased significantly,while that of Basidiomycota and Mortierel-lomycota decreased significantly.At genus level,the relative abundance of Chaetomium increased significant-ly,while that of Mortierella,Tausonia and Plectosphaerella decreased significantly.B.subtilis could effectively reduce the incidence of strawberry root rot with the control effect higher than 70%within 2 months of planting,and significantly increased the growth indexes such as canopy diameter,leaf area and chlorophyll content.Comprehensive analysis showed that B.subtilis could increase the relative abundance of beneficial fungi but re-duce that of pathogenic fungi in strawberry rhizosphere soil,so optimized the soil fungal community structure,effectively controlled strawberry root rot and promoted the healthy growth of strawberry.关键词
枯草芽孢杆菌/草莓/高通量测序/真菌群落结构/草莓根腐病Key words
Bacillus subtilis/Strawberry/High-throughput sequencing/Fungi community structure/Strawberry root rot分类
农业科技引用本文复制引用
李宝庆,石亚冰,凌凡舒,徐丹,孙卿,刘玉珊,韩庆典..枯草芽孢杆菌对土壤真菌群落结构及草莓根腐病的调控作用[J].山东农业科学,2024,56(6):81-87,7.基金项目
山东省生态农业产业技术体系项目(SDAIT-30-12) （SDAIT-30-12）
山东省农业重大应用技术创新项目(2015NY212) （2015NY212）
浙江大学山东(临沂)现代农业研究院服务地方经济发展项目(ZDNY-2021-FWLY02004) （临沂）
临沂大学社会服务项目(HX230195) （HX230195）
