实用医学杂志2024，Vol.40Issue(13)：1791-1796,6.DOI:10.3969/j.issn.1006-5725.2024.13.005
miR-155通过SOCS1/STAT3途径调控类风湿性关节炎中炎症反应和Th17/Treg失衡
MiR-155 regulates inflammatory responses and Th17/Treg imbalances in rheumatoid arthritis through the SOCS1/STAT3 pathway
摘要
Abstract
Objective This research aimed to investigate the expression and mechanism of miR-155 and suppressor of cytokine signaling 1(SOCS1)in rheumatoid arthritis(RA).Methods RT-PCR and flow cytometry were applied to detect the expression differences of miR-155,Th17,and Treg cells in peripheral blood of RA patients(RA group)and control group(HC group).Bioinformatics analysis and dual-luciferase reporter assay were conducted to investigate the regulatory relationship between miR-155 and SOCS1.CD4+T cells were isolated from peripheral blood of RA patients and transfected with miR-155 inhibitor,si-SOCS1,and their respective negative control sequences,and divided into four groups:miR-NC group,miR-155 inhibitor group,miR-155 inhibitor+si-NC group,and miR-155 inhibitor+si-SOCS1 group.The cells were treated with Th17-inducing differentiation medium,and flow cytometry was used to determine the ratio of Th17 cells in each group of CD4+T cells.Western blot was used to determine the ratio of p-STAT3/STAT3 in the cells.Results Compared to the HC group,RA patients showed increased expression of miR-155 and Th17 ratio(P<0.01),and decreased Treg cell ratio(P<0.01).MiR-155 could target and inhibit the expression of SOCS1.Compared to the miR-NC group,the miR-155 inhibitor group,miR-155 inhibitor+si-NC group,and miR-155 inhibitor+si-SOCS1 group showed decreased Th17 ratio and p-STAT3/STAT3 ratio(P<0.01).Compared to the miR-155 inhibitor group,the miR-155 inhibitor+si-SOCS1 group exhibited increased Th17 ratio and p-STAT3/STAT3 ratio in CD4+T cells(P<0.05).Conclusion Elevated expression of miR-155 in RA patients may mediate the differentiation of CD4+T cells into Th17 cells through the SOCS1/STAT3 pathway,contributing to the imbalance of Th17/Treg cells in peripheral blood of RA patients.关键词
类风湿性关节炎/miR-155/细胞因子信号转导抑制因子1/Th17/Treg失衡Key words
rheumatoid arthritis/miR-155/suppress of cytokine signaling 1(SOCS1)/Th17/Treg imbalance分类
医药卫生引用本文复制引用
张玉红,单新洁,周俊..miR-155通过SOCS1/STAT3途径调控类风湿性关节炎中炎症反应和Th17/Treg失衡[J].实用医学杂志,2024,40(13):1791-1796,6.基金项目
兵团科技攻关与成果转化计划项目(编号:2020AD008) （编号:2020AD008）
