实用医学杂志2024，Vol.40Issue(13)：1814-1821,8.DOI:10.3969/j.issn.1006-5725.2024.13.009
肺癌合并肺结核的蛋白组学图谱及差异表达蛋白功能分析
Proteomic profiling and functional analysis of differentially expressed proteins in lung cancer coexistent with pulmonary tuberculosis
摘要
Abstract
Objective This study aims to analyze the proteomic characteristics of peripheral blood in patients with lung cancer coexistent with pulmonary tuberculosis(LC-PTB),identify the differential proteins compared with lung cancer(LC)patients,and conduct functional analysis on these proteins.Methods The study included 8 LC-PTB patients and 10 LC patients.The LC patients were newly diagnosed and confirmed by pathology and did not receive any anti-tumor treatment before,while the PTB patients were Mycobacterium tuberculosis positive at the time of sampling.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)was applied to perform proteomic mass spectrometry to assess the differential proteins,and then functional analysis was conducted via bioinformatics.Results A total of 5,185 proteins were detected between two groups.Through differential expression screening,190 proteins(58 upregulated and 132 downregulated)were identified to be differentially expressed.Subcellular localization analysis revealed that the differential proteins were mainly concentrated in the cytoplasm,nucleus,and extracellular matrix.KEGG pathway and GO analysis showed the roles of differential proteins in biological processes including immune response,metabolism,and secretion regulation.Protein interaction network analysis highlighted the importance of SORT1,SAR1B,RPS6KB1,VWF,SHC1,SRPRB,CTSD,TARDBP,RPLP0,PSMA2,RPS6,XPO1,PRKACB,and HLA-DRB1 in LC-PTB.Additionally,the expression changes in proteins like ADA2,MAP3K1,and GLS2 might be closely associated with the development of LC-PTB.Conclusions The proteomic profile comprehensively described the proteomic characteristics of LC-PTB and identified numerous differentially expressed proteins,which could provide further clues for research on biological mechanism of LC-PTB.关键词
肺癌/肺结核/蛋白质组学/液相色谱-质谱串联技术Key words
lung cancer/pulmonary tuberculosis/proteomics/liquid chromatography-tandem mass spectrometry分类
医药卫生引用本文复制引用
周文娣,林佳敏,琚岱晨,王琪,朱家楼,苏宁,胡锦兴..肺癌合并肺结核的蛋白组学图谱及差异表达蛋白功能分析[J].实用医学杂志,2024,40(13):1814-1821,8.基金项目
国家重点研发计划(编号:2022YFC2304800) （编号:2022YFC2304800）
广州市科技计划项目(编号:2023A03J0539,2023A03J0992,2023A03J0534) （编号:2023A03J0539,2023A03J0992,2023A03J0534）
