实用医学杂志2024，Vol.40Issue(13)：1785-1790,6.DOI:10.3969/j.issn.1006-5725.2024.13.004
大黄素上调Sirt2减轻脂多糖致RAW264.7细胞的氧化应激反应
Emodin upregulates the Sirt2 to attenuate LPS-induced oxidative stress response in RAW264.7 cells
摘要
Abstract
Objective The aim of this study was to investigate the impact and mechanisms of emodin on oxidative stress response in lipopolysaccharide(LPS)-induced murine mononuclear macrophages(RAW264.7).Methods Involved the use of LPS,RAW264.7 cells,and emodin.Experimental groups included a control group,LPS(1 μg/mL)group,and LPS(1 μg/mL)+emodin(15 μmmol/L)pretreatment group.Aldehyde malondialdehyde(MDA)content,intracellular reactive oxygen species(ROS)levels,and silent information regulator 2(Sirt2)expression were evaluated at 6,12,and 18 hours after LPS exposure.Additionally,RAW264.7 cells were pretreated with Sirt2 inhibitor AGK2(20 μmol/L)followed by LPS stimulation,and the above-mentioned parameters were assessed at 6 hours.Results Compared to the control group,MDA content,ROS levels,Sirt2 mRNA,and protein expression in RAW264.7 cells in the LPS group increased at all time points(all P<0.05).At 6 and 18 hours,MDA content and ROS levels in RAW264.7 cells in the LPS+emodin group decreased significantly(all P<0.05),while at 12 hours,ROS levels were lower in the LPS group compared to the LPS+emodin group(P<0.05).Sirt2 mRNA and protein levels significantly increased at all time points(all P<0.05)compared to the LPS group.In the LPS+emodin+AGK2 group,Sirt2 mRNA and protein levels decreased,and MDA content and ROS levels increased compared to the LPS+emodin group(all P<0.05).Conclusion LPS-induced oxidative stress in RAW264.7 cells and emodin attenuate LPS-induced oxidative stress in RAW264.7 cells through Sirt2.关键词
脓毒症/巨噬细胞/氧化应激/大黄素/Sirt2Key words
sepsis/macrophages/oxidative stress/emodin/Sirt2分类
医药卫生引用本文复制引用
龙淳,毕红英,杨昌珍,王家锴,唐艳,刘旭..大黄素上调Sirt2减轻脂多糖致RAW264.7细胞的氧化应激反应[J].实用医学杂志,2024,40(13):1785-1790,6.基金项目
国家重点研发计划(编号:2018YFC2001904) （编号:2018YFC2001904）
国家自然科学基金项目(编号:81960357,81701958) （编号:81960357,81701958）
国家自然科学基金培育项目(编号:Gyfynsfc[2020]-26) （编号:Gyfynsfc[2020]-26）
贵州省科技计划项目(编号:黔科合基础[2020]1Y330,黔科合基础-ZK[2022]一般370,黔科合支撑[2021]一般090) （编号:黔科合基础[2020]1Y330,黔科合基础-ZK[2022]一般370,黔科合支撑[2021]一般090）
