生态学报2024，Vol.44Issue(12)：5269-5279,11.DOI:10.20103/j.stxb.202301190124
锰矿区大叶胡枝子灌丛土壤的丛枝菌根真菌多样性
Diversity of arbuscular mycorrhizal fungi in the soil of Lespedeza davidii scrub in manganese mining area
摘要
Abstract
Lespedeza davidii is a shrub belonging to Fabaceae,which is also an important phytoremediation candidant plant in mining areas because of its strong tolerance to heavy metal stress and the ability of nitrogen-fixation.Arbuscular mycorrhizal fungi(AMF)is a kind of symbiotic fungi for plants,and its symbiosis with plants in the mining area can promote the plant growth and improve the plant resistance to stress.However,its species composition,distributions and influencing factors are still unclear.The AMF community of L.davidii scrub soil was selected as the study object,and the soil under L.davidii canopy and adjacent open land in mining and non-mining areas were collected to carry out internal transcribed spacer(ITS)sequencing and physical and chemical characteristics,aiming to reveal the diversity of soil AMF community in different sample sites and its influencing factors.The results showed that:(1)A total of 2961 operational taxonomic units(OTUs)were obtained from soil samples,among which 66 OTUs belonged to AMF.By consulting Silva database,7 orders,10 families,16 genera and 24 AMF species were obtained.Total species abundance of AMF was the soil in the adjacent open land of mining area＞soil under the crown of mining area＞soil under the crown of non-mining area＞soil in the adjacent open land of non-mining area.The trend of Shannon Index and Simpson Index was the same as that of species abundance.(2)Entrophospora and so on were the dominant genera in the AMF community of the soil in mining area,while Funneliformis and so on were the dominant genera in the AMF community of the soil under the crown of non-mining area.Entrophospora infrequens was the common dominant species in four sample sites,which belonged to a broad-spectrum ecotype species.Archaeospora sp.and Paraglomerales sp.were the dominant species in the AMF community of the soil under the crown of mining area,with strong adaptability to heavy metal stress.Funneliformis sp.was the dominant species in the AMF community of the soil under the crown of non-mining area.(3)Redundancy analysis(RDA)illustrated that the species and amounts of AMF were significantly affected by Mn content,soil pH value,and total phosphorus content.All these results showed that AMF species and diversity were effected by soil physicochemical properties.The presence of some AMF,which had strong tolerance in mining area,improved the species diversity,and they can help plant grow under adverse conditions.关键词
矿区/大叶胡枝子/丛枝菌根真菌/多样性/灌丛Key words
mining area/Lespedeza davidii/arbuscular mycorrhizal fungi/diversity/scrub引用本文复制引用
王雯颖,徐宝珠,胡佳瑶,潘高,刘文胜..锰矿区大叶胡枝子灌丛土壤的丛枝菌根真菌多样性[J].生态学报,2024,44(12):5269-5279,11.基金项目
国家自然科学基金(42177018) （42177018）
湖南省自然科学基金(2022JJ40193) （2022JJ40193）
