Apelin对滋养细胞生物学功能的影响及对子痫前期样小鼠的治疗作用OACSTPCD

Objective:To explore the effect of Apelin on the biological functions of trophoblast cells and its therapeutic effect on preeclampsia-like mice.Methods:Western blot was used to detect the expression level of Apelin protein in placenta tissue of normal pregnant women and preeclampsia patients.RT-qPCR and Western blot were used to detect the expres-sion levels of Apelin mRNA and protein in trophoblast cells.Cell immunofluorescence was used to detects the localization of Apelin in trophoblast cells.Label-free cell proliferation experiment,colony formation experiment and EdU staining were used to detect the effect of Apelin on the proliferation ability of trophoblast cells.Cell scratching and transwell chamber were used to de-tect the effect of Apelin on the migration and invasion ability of trophoblast cells.Blood vessel formation experiments were performed to detect the effect of Apelin on the blood vessel forma-tion ability of trophoblast cells.Western blot was used to detect matrix metalloproteinase 2(MMP-2),matrix metalloproteinase 9(MMP-9),N-cadherin,E-cadherin,Erk,and P-Erk pro-tein expression levels in the control group,Apelin stimulation group and Apelin inhibitor group.By constructing a preeclampsia-like mouse model and giving Apelin injection treatment,the sys-tolic blood pressure and urine protein levels of mice in each group were detected,as well as the proportion of live fetuses,fetal weight,placental weight and the ratio between the two.Result:The expression of Apelin in the placental tissue of preeclampsia patients was reduced.Apelin was expressed and localized in the cytoplasm of trophoblasts.In terms of cell biological func-tion,Apelin had no effect on the proliferation ability of trophoblasts,but promoted the migration and invasion ability of trophoblasts by up-regulating the expressions of MMP-2 and N-cadherin.Angiogenesis experiments proved that Apelin could promote the vascularization ability of tropho-blasts.Western blot showed that Apelin promoted the phosphorylation level of Erk protein in the MAPK/Erk pathway,and the results of the preeclampsia mouse model showed that Apelin could alleviate its clinical symptoms and improve the embryonic survival and development level of mice.Conclusion:Apelin is expressed in the placental tissue of preeclampsia patients,and can upregulate the migration,invasion and vascularization ability of trophoblasts.Apelin has a thera-peutic effect on preeclampsia-like mice,which may become a potential therapeutic target for preeclampsia.