湖北科技学院学报（医学版）2024，Vol.38Issue(4)：277-281,5.DOI:10.16751/j.cnki.2095-4646.2024.04.0277
白及多糖对脂多糖诱导的小鼠急性肝损伤的作用及其机制研究
Effect of the Bletilla Striata Polysaccharide on Lipopolysaccharide-Induced Acute Liver Injury in Mice and its Mechanisms
葛婷婷 1陈祺 1王洁琼 1杨洪飞 1卢雨菲 1孟丽媛 1要辉 1闵清 2白育庭3
作者信息
- 1. 湖北科技学院医学部药学院,湖北 咸宁 437100
- 2. 湖北科技学院医学部药学院,湖北 咸宁 437100||鄂南特色中药湖北省工程研究中心
- 3. 湖北科技学院医学部临床医学院
- 折叠
摘要
Abstract
Objective To study the effect and mechanism of Bletilla striata polysaccharide(BSP)on lipopolysaccharide(LPS)-induced acute liver injury in mice.Methods Sixty Kunming male mice were randomly divided into control group,model group(LPS 1mg/kg,Model group),positive control group(2mg/kg BifendatePills,BDP group),low,medium and high dose BSP administration groups(BSP 100,200,400mg/kg group).The control group and the model group were given normal saline,and the other four groups were given the corresponding drug dose once a day for 7 days.Two hours after the last administration,except for the control group,the other five groups were intraperitoneally injected with LPS(1mg/kg)to establish the model of acute liver inju-ry.Twelve hours after the modeling,liver and plasma were corrected.Subsequently,the liver index was calculated,and the contents of AST,ALT,MDA,SOD,GSH-Px,and CAT in plasma were measured.The expression levels of TLR4/MyD88/NF-κB and TNF-α/IL-1β/IL-6 proteins in the liver tissue of mice in each group were detected by Western blotting.Results Compared with the control group,the liver index of the model group was significantly increased(P<0.01),the contents of AST,ALT and MDA in plasma were increased(P<0.01),and the contents of SOD,GSH-Px and CAT were significantly decreased(P<0.01).The structure of liver lobule was not clear in the model group accompanied by a large number of inflammatory cell infiltration.Compared with the model group,BSP intervention significantly reduced the liver index,increased the contents of SOD,GSH-Px and CAT(P<0.01),and significantly decreased the contents of AST,ALT and MDA in plasma(P<0.01).The protein expressions of TLR4/MyD88/NF-κB and TNF-α/IL-1β/IL-6 were significantly down-regulated(P<0.05).Conclusion BSP has a protective effect on LPS-in-duced acute liver injury in mice,and its mechanisms may be related to antioxidant and inhibition of TLR4/MyD88/NF-κB signa-ling pathway proteins and reduction of the expression of inflammatory factors such as TNF-α/IL-1β/IL-6.关键词
白及多糖/脂多糖/急性肝损伤Key words
Bletilla striata Polysaccharide/Lipopolysaccharide/Acute Liver Injury分类
医药卫生引用本文复制引用
葛婷婷,陈祺,王洁琼,杨洪飞,卢雨菲,孟丽媛,要辉,闵清,白育庭..白及多糖对脂多糖诱导的小鼠急性肝损伤的作用及其机制研究[J].湖北科技学院学报（医学版）,2024,38(4):277-281,5.
