湖北科技学院学报（医学版）2024，Vol.38Issue(4)：287-291,296,6.DOI:10.16751/j.cnki.2095-4646.2024.04.0287
阿苯达唑对人食管鳞癌细胞KYSE-150的抑制作用
Inhibitory Effect of Albendazole on Human Esophageal Squamous Cell Carcinoma Cells KYSE-150
蔡敬 1贾爱亭 1曾凤娇 1赵冰洁 1宁志丰2
作者信息
- 1. 湖北科技学院医学部药学院,湖北 咸宁 437100
- 2. 湖北科技学院医学部基础医学院
- 折叠
摘要
Abstract
Objective To investigate the inhibitory effect and mechanism of albendazole on esophageal squamous cell carci-noma KYSE-150 cells.Methods The proliferation ability of KYSE-150 cells was evaluated by MTT assay and colony formation as-say.The migration and invasion ability of KYSE-150 cells were demonstrated by wound healing assay and Transwell chamber as-say.Meanwhile,the apoptosis and necrosis detection kit was used to detect whether albendazole could induce apoptosis of KYSE-150 cells,and the expression levels of apoptosis-related proteins BAX,Bcl-2 and P53 were investigated by Western blot.Results Albendazole inhibited the viability,proliferation,migration and invasion of KYSE-150 cells in a dose-dependent manner.The results of YO-PRO-1/PI double staining showed that the administration could induce cell apoptosis,and at the same time,it caused the down-regulation of the expression of Bcl-2 protein,while the up-regulation of the expression of BAX and P53 protein.Conclusion Albendazole has a significant inhibitory effect on the proliferation and migration of esophageal squamous carcinoma KYSE-150 cells,and the mechanism may be related to the regulation of apoptosis-related proteins.关键词
阿苯达唑/食管鳞癌/迁移/凋亡Key words
Albendazole(ABZ)/Esophageal cancer/Migration/Apoptosis分类
医药卫生引用本文复制引用
蔡敬,贾爱亭,曾凤娇,赵冰洁,宁志丰..阿苯达唑对人食管鳞癌细胞KYSE-150的抑制作用[J].湖北科技学院学报（医学版）,2024,38(4):287-291,296,6.
