湖北科技学院学报（医学版）2024，Vol.38Issue(4)：292-296,5.DOI:10.16751/j.cnki.2095-4646.2024.04.0292
双吗啉取代吡啶锌和铜配合物的合成、表征及抗癌活性初步研究
Preliminary Studies of Synthesis,Characterization and Anticancer Activity of Zinc and Copper Complexes Based on Bis-Morpholine Substituted Pyridine
摘要
Abstract
Objective To research the synthesis and anticancer activities of zinc and copper complexes based on bis-mor-pholine substituted pyridine(Mor2-Py-Zn and Mor2-Py-Cu).Methods Taking the antagonist AMD3100,which entered the clinical trial,as the lead compound,Mor2-Py-Zn and Mor2-Py-Cu were designed and synthesized,and characterized by high resolution mass spectrometry.The crystal structure was determined by X-ray single crystal diffraction.The interaction between complexes and calf thymus DNA(CT-DNA)was studied by UV-Vis absorption spectroscopy,and the cytotoxicity against glioblastoma cells was further studied by morphological analysis and MTT assays.Results Mor2-Py-Zn belongs to the orthorhombic system,pbca space group,pyr-idine N,two morpholine N and two nitrate O atoms form a triangular bipyramidal configuration of N3O2 pentagram with Zn2+.Both Mor2-Py-Zn and Mor2-Py-Cu interacted with CT-DNA in solution to some extent.The two complexes showed weak inhibitory activi-ty against U87,but obviously inhibited the proliferation of U251.Further,MTT assay showed that the IC50 of Mor2-Py-Zn and Mor2-Py-Cu for U251 cells were 18.11μmol/L and 14.66 μmol/L,respectively.Conclusion Both Mor2-Py-Zn and Mor2-Py-Cu are val-uable for further study of their anticancer mechanism,especially for U251 cells.关键词
锌配合物/铜配合物/抗癌/人胶质瘤细胞/DNA相互作用/晶体结构Key words
Zinc complex/Copper complex/Anticancer/Glioma cells/Interaction with DNA/Crystal structure分类
医药卫生引用本文复制引用
汪清清,潘耀,游双,黄鑫,熊衍才,王小波..双吗啉取代吡啶锌和铜配合物的合成、表征及抗癌活性初步研究[J].湖北科技学院学报（医学版）,2024,38(4):292-296,5.基金项目
湖北省教育厅重点项目(D20212802) （D20212802）
