湖北科技学院学报（医学版）2024，Vol.38Issue(4)：301-305,5.DOI:10.16751/j.cnki.2095-4646.2024.04.0301
基于PBNPs-Ag纳米粒子的ROS清除和抗菌协同作用促进伤口愈合行为研究
Study on the ROS Scavenging and Antibacterial Synergistic Effect of PBNPs-Ag Nanoparticles for Wound Healing
唐冬旭 1刘坤 1付娌丽 1周士忠 1张洋林 1李月生2
作者信息
- 1. 湖北科技学院医学部药学院,湖北 咸宁 437100
- 2. 湖北科技学院辐射化学与功能材料湖北省重点实验室||湖北科技学院核技术与化学生物学院
- 折叠
摘要
Abstract
Objective To combine Prussian blue nanoparticles(PBNPs)and silver nanoparticles(Ag)to synthesize PB-NPs-Ag nanoparticles with both ROS scavenging and antibacterial effects for promoting wound healing.Methods X-ray diffraction(XRD),infrared spectroscopy(IR),Zeta potential analysis,particle size measurements were employed to characterize the physico-chemical properties of PBNPs-Ag.At the cellular level,the cytotoxicity of PBNPs-Ag was tested using the MTT assay followed by establishing a cellular model for ROS prevention utilizing H2 O2 as a ROS initiator.Additionally,DCFH-DA staining was performed for direct visualization of the intracellular ROS levels.Finally,an inhibition circle assay was conducted to evaluate the inhibitory effect of PBNPs-Ag on bacteria.Results The synthesized PBNPs-Ag nanoparticles exhibited uniform particle size distribution,high crystallinity and negligible cytotoxicity.Moreover,they demonstrated excellent capability in scavenging ROS and antibacterial abili-ties.Conclusion PBNPs-Ag is an effective and promising nanoparticle drug for the treatment of skin injuries.关键词
普鲁士蓝/银纳米粒子/ROS清除/抗菌/皮肤损伤Key words
Prussian blue/Silver nanoparticles/ROS scavenging/Antibacterial/Skin injuries分类
医药卫生引用本文复制引用
唐冬旭,刘坤,付娌丽,周士忠,张洋林,李月生..基于PBNPs-Ag纳米粒子的ROS清除和抗菌协同作用促进伤口愈合行为研究[J].湖北科技学院学报（医学版）,2024,38(4):301-305,5.
