针刺研究2024，Vol.49Issue(7)：678-685,8.DOI:10.13702/j.1000-0607.20230990
电针"足三里"联合capeOX方案对结直肠癌裸鼠肿瘤细胞凋亡和铁死亡的影响
Effect of electroacupuncture of"Zusanli"(ST36)combined with capeOX on apoptosis and ferroptosis in nude mice with colorectal cancer
摘要
Abstract
Objective To investigate the impact of combined treatment of colorectal cancer(CRC)with electroacupuncture(EA)and capeOX(combined administration of fluorouracil,oxaliplatin and capecitabine)on the tumor volume,weight,spleen coefficient,apoptosis and ferroptosis of tumor tissue,and liver and kidney functions in nude mice with CRC,so as to explore its mechanisms underlying inhibiting CRC and alleviating toxic reactions of capeOX.Methods Female Balb/c nude mice were randomly assigned to 3 groups:model,capeOX,and EA+capeOX,with 8 nude mice in each group.The CRC model was established by subcutaneous injection of colon cancer cells at the right inguinal region.Nude mice of the capeOX group received intraperitoneal injection of oxaliplatin for 1 day and gavage of capecitabine from day 2 to day 7.EA(1 mA,2 Hz/100 Hz)was applied to bilateral"Zusanli"(ST36)for 20 min,once daily for 7 days.During the interven-tion,the tumor volume and weight were measured every day,and at the end of intervention,the weight of the tumor tissue and spleen were measured,with tumor volume difference and spleen coefficient calculated.The proportion of apoptotic cells was measured by flow cytometry,and the contents of serum malondialdehyde(MDA),alanine aninotransferase(ALT),aspartate aminotransferase(AST),blood urea nitrogen(BUN),and creatinine(Cr)were detected using ELISA.The expression level of glutathione peroxidase 4(GPX4,a key regulator for ferroptosis)protein of the tumor tissue was determined using Western blot.Results Compared to the model group,both the capeOX group and EA+capeOX group showed a decrease in the tumor volume(on day 3 and 4 in the capeOX group,and from day 2 to 7 in the EA+capeOX group)and body weight(P<0.05,on day 3 to 7 in the EA+capeOX group and on day 2 to 7 in the capeOX group),being evidently lower in the tumor volume on day 7 in the EA+capeOX than in the capeOX group(P<0.05),and evidently higher in the body weight on day 6 and 7 in the EA+capeOX group than in the capeOX group(P<0.05).In comparison with the model group,the tumor volume difference,tumor weight and spleen coefficient in both capeOX and EA+capeOX groups were significantly decreased(P<0.05),and MDA content in EA+capeOX group was significantly decreased(P<0.05),while the contents of ALT,BUN and Cr in the capeOX group,the proportion of apoptotic cells in both capeOX and EA+capeOX groups,and the GPX4 expression level in the EA+capeOX group were all significantly increased(P<0.05).The tumor volume difference,tumor weight,and contents of MDA,ALT,AST,BUN and Cr in the EA+capeOX group were markedly lower than in the capeOX group(P<0.05),while the spleen coefficient,proportion of apoptotic cells and GPX4 expression level in the EA+capeOX group were markedly higher than those in the capeOX group(P<0.05).Conclusion EA of ST36 can enhance the effect of capeOX in inhibiting colorectal cancer growth in nude mice with CRC,which may be related with its functions in promoting tumor cell apoptosis,inhibiting ferroptosis,and modulating immune tolerance.In addition,EA can lower the side effects of capeOX in hematopoietic and immune,liver,and kidney functions.关键词
电针/capeOX方案/足三里/结直肠癌/凋亡/铁死亡Key words
Electroacupuncture/CapeOX/Zusanli(ST36)/Colorectal cancer/Apoptosis/Ferroptosis引用本文复制引用
李泽丽,曾文静,李国海,张洁桐,李祉澄,王思颖,邱芳华,李述文..电针"足三里"联合capeOX方案对结直肠癌裸鼠肿瘤细胞凋亡和铁死亡的影响[J].针刺研究,2024,49(7):678-685,8.基金项目
广东省自然科学基金面上项目(No.2021A1515010668) （No.2021A1515010668）
市校(院)企联合资助专题项目(No.2023A03J0314) （院）
