"扶正益肝"针灸穴方基于肌醇需求酶1信号通路改善顺铂致小鼠肝损伤的机制OA北大核心CSTPCDMEDLINE

Objective To investigate the mechanism of the effect of acupuncture and moxibustion on improving liver injury in cisplatin(DDP)induced liver injury model mice by observing the changes of inositol-requiring enzyme(IRE)-1 signaling pathway.Methods Forty KM mice were randomly divided into control,model,acupuncture and moxibustion groups,with 10 mice in each group.The liver injury model was replicated by intraperitoneal injection of DDP(10 mg/kg).In the acupuncture group and the moxibustion group,acupuncture and moxibustion were performed at"Dazhui"(GV14),and bilateral"Ganshu"(BL18),"Shenshu"(BL23),and"Zusanli"(ST36),respectively for 6 min,once per day for 7 d.The apoptosis of hepatocytes was detected by TUNEL staining.The expression of phosphorylation(p)-IRE-1α,glucose-regulated protein(Grp)78 and cysteine aspartic protease(Caspase)-12 in liver tissue were detected by immunohistochemistry and Western blot,respectively.The expression levels of Grp78 and Caspase-12 mRNA in liver tissue were detected by quantitative real-time PCR.Results Compared with the control group,the apoptosis rate of hepatocytes was increased(P<0.05),the positive expression and protein expression of p-IRE-1α,Grp78,and Caspase-12 were increased(P<0.05),the expression levels of Grp78 and Caspase-12 mRNA were increased(P<0.05)in the model group.Compared with the model group,all these indicators showed opposite trends(P<0.05)in the acupuncture and moxibustion groups.Conclusion Acupuncture and moxibustion can reduce liver injury due to DDP chemotherapy by modulating IRE-1 signaling pathway,inhibiting the excessive activation of endoplasmic reticulum stress,and reducing liver cell apoptosis.