针刺研究2024，Vol.49Issue(7)：686-692,7.DOI:10.13702/j.1000-0607.20240125
"扶正益肝"针灸穴方基于肌醇需求酶1信号通路改善顺铂致小鼠肝损伤的机制
Mechanism of acupuncture and moxibustion in ameliorating liver injury induced by cisplatin by regulating IRE-1 signaling pathway
摘要
Abstract
Objective To investigate the mechanism of the effect of acupuncture and moxibustion on improving liver injury in cisplatin(DDP)induced liver injury model mice by observing the changes of inositol-requiring enzyme(IRE)-1 signaling pathway.Methods Forty KM mice were randomly divided into control,model,acupuncture and moxibustion groups,with 10 mice in each group.The liver injury model was replicated by intraperitoneal injection of DDP(10 mg/kg).In the acupuncture group and the moxibustion group,acupuncture and moxibustion were performed at"Dazhui"(GV14),and bilateral"Ganshu"(BL18),"Shenshu"(BL23),and"Zusanli"(ST36),respectively for 6 min,once per day for 7 d.The apoptosis of hepatocytes was detected by TUNEL staining.The expression of phosphorylation(p)-IRE-1α,glucose-regulated protein(Grp)78 and cysteine aspartic protease(Caspase)-12 in liver tissue were detected by immunohistochemistry and Western blot,respectively.The expression levels of Grp78 and Caspase-12 mRNA in liver tissue were detected by quantitative real-time PCR.Results Compared with the control group,the apoptosis rate of hepatocytes was increased(P<0.05),the positive expression and protein expression of p-IRE-1α,Grp78,and Caspase-12 were increased(P<0.05),the expression levels of Grp78 and Caspase-12 mRNA were increased(P<0.05)in the model group.Compared with the model group,all these indicators showed opposite trends(P<0.05)in the acupuncture and moxibustion groups.Conclusion Acupuncture and moxibustion can reduce liver injury due to DDP chemotherapy by modulating IRE-1 signaling pathway,inhibiting the excessive activation of endoplasmic reticulum stress,and reducing liver cell apoptosis.关键词
针灸/顺铂/肝损伤/内质网应激/肌醇需求酶-1αKey words
Acupuncture and moxibustion/Cisplatin/Liver injury/Endoplasmic reticulum stress/IRE-1α引用本文复制引用
于冬冬,王建明,韩乐,杜雪源,晁利芹,张欢欢,吴昊,王晴晴,刘文姣.."扶正益肝"针灸穴方基于肌醇需求酶1信号通路改善顺铂致小鼠肝损伤的机制[J].针刺研究,2024,49(7):686-692,7.基金项目
国家自然科学基金青年基金项目(No.81804196) （No.81804196）
河南省省级科技研发计划联合基金项目(优势学科培育类)(No.222301420077) （优势学科培育类）
河南中医药大学2023年度研究生科研创新项目(No.2023KYCX074) （No.2023KYCX074）
