中国农业科技导报2024,Vol.26Issue(7):37-49,13.DOI:10.13304/j.nykjdb.2023.0183
老山芹层积前后种子差异代谢物分析
Analysis of Differential Seed Metabolites Before and After Stratification of Heracleum moellendorffii Hance
摘要
Abstract
In order to deeply explore the seed dormancy mechanism of Heracleum moellendorffii Hance,the different metabolites of non-lamellarized seeds(NS)and germination seeds after lamellarization(GS)were analyzed by GC-TOF/MS(gas chromatography-time of flight mass spectrometry).The results showed that a total of 995 metabolites were detected,and there was a clear separation between NS and GS.There were 126 differential metabolites,among which 75 were extremely significant differential metabolites including 35 up-regulated and 40 down-regulated expressions.The metabolic pathways of the extremely significant differential metabolites were analysed and 10 key metabolic pathways were identified.The results of the chord diagram analysis showed that the lipids(including lipid-like molecules)was extremely significantly positive correlaed with benzenoids,while the benzenepropanoids(including polyketides)was extremely significantly negative correlation with organoxides.Above results provided theoretical basis of metabolomics for exploring the post-maturation germination of Heracleum moellendorffii Hance seeds,and researching the post-maturation and dormancy mechanisms of Heracleum moellendorffii Hance seeds.关键词
老山芹/GC-TOF/MS/代谢组学/层积/差异代谢物Key words
Heracleum moellendorffii Hance/GC-TOF/MS/metabolomics/stratification/differential metabolites分类
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张永芳,张宏发,于萍,李富恒,董世妍,王佳轩,郭绪虎,张畅,王艳星,王钰烨,吴静凤,鲍甜芳..老山芹层积前后种子差异代谢物分析[J].中国农业科技导报,2024,26(7):37-49,13.基金项目
国家自然科学基金项目(31801872) (31801872)
山西省高等学校科技创新计划项目(2022L459) (2022L459)
山西大同大学人文社科项目(2022YGZX005) (2022YGZX005)
大同市科技攻关项目(2020044) (2020044)
大同大学产学研项目(2020CXZ14). (2020CXZ14)