中国麻业科学2024，Vol.46Issue(3)：169-174,6.
酿酒酵母表面展示NX1101蛋白酶全细胞催化剂制备火麻多肽
Preparation of Industrial Hemp Seed Polypeptide by a Whole-cell Biocatalyst Displayed NX1101 Protease on the Surface of Saccharomyces cerevisiae
摘要
Abstract
The preparation of industrial hemp seed polypeptide by enzymatic hydrolysis has the ad-vantages of good safety,low energy consumption and little nutritional loss.In this study,based on the synthesis of defatted hemp seed meal protein extraction and proteolytic enzyme screening,the shuttle plasmid pYD1 was used as the expression vector to anchor proteolytic enzyme NX1101 from bovine intes-tinal metgenomic library on the surface of Saccharomyces cerevillus(EYB100),and the recombinant strain was used as the whole cell catalyst to prepare hemp polypeptides.The enzymatic properties analysis of the whole cell catalyst showed that the most suitable reaction pH value was 8.0,and the enzyme activi-ty stability was high between 8 and 10,so it was an alkaline resistant protease.The optimum reaction temperature was 50℃,and the stability of enzyme activity was higher below 50℃.A total of 1095 pep-tides were obtained by enzymolysis of the defatted hemp protein with the whole cell catalyst of recombi-nant yeast displaying NX1101,among which 140 short peptides with amino acid number≤10 were ob-tained.The enzymolysis efficiency of whole cell catalyst was more than 6.5 times higher than that of con-ventional enzymolysis technology with alkaline protease powder.关键词
火麻肽/蛋白水解酶/酶学性质/全细胞催化剂/表面展示Key words
hemp peptide/proteolytic enzyme/enzymatic property/whole cell catalyst/surface display分类
农业科技引用本文复制引用
曲亚通,陈继康,苏宇波,王悦,高钢,赵浩含,陈佳,王晓飞,马智峰,朱爱国..酿酒酵母表面展示NX1101蛋白酶全细胞催化剂制备火麻多肽[J].中国麻业科学,2024,46(3):169-174,6.基金项目
财政部和农业农村部:国家麻类产业技术体系建设专项(CARS-16) （CARS-16）
甘肃省技术创新引导计划"张家川工业大麻提质增效关键技术研发与集成示范"(22CX8NE213) （22CX8NE213）
