中国农业科技导报2024，Vol.26Issue(7)：121-135,15.DOI:10.13304/j.nykjdb.2022.1006
油茶根腐病根际土壤、根系内真菌群落结构和多样性分析
Analysis on Structure and Diversity of Fungi Community in Rhizosphere Soil and Root System of Camellia oleifera Root Rot
摘要
Abstract
To clarify the fungal community composition,diversity,functional characteristics and environmental factor relevance in the root system and inter-rhizosphere soil of Camellia oleifera with root rot at different disease levels,internal transcribed spacer(ITS)sequences of fungi within the fibrous roots and rhizosphere soil of C.oleifera of 5 disease classes were sequenced using high-throughput sequencing technology in Lianghe county,Dehong prefecture.The results showed that the diversity(Shannon index)of fungal communities within the diseased C.oleifera fibrous roots and rhizosphere soil would decrease,and the total operational taxonomic units(OTUs),endemic OTUs and abundance(Chao1 index)of fungi in the roots showed an increasing trend followed by a decreasing trend;the uniformity and abundance of fungal communities in the rhizosphere soil of diseased C.oleifera increased.At the phylum level,Ascomycota and Basidiomycota were the common dominant phylum in the fibrous roots and rhizosphere soil,while Glomeromycota and Mortierellomycota showed a decreasing trend in abundance in the fibrous roots.At the genus level,the abundance of Dendrosporium,Cordana,Matsushimamyces,Pezicula and Melanconium increased significantly after the disease.The main influenced factors of soil fungal community structure in C.oleifera understory were available potassium,organic matter,pH and available phosphorus,and the results of combined analysis of fungal community and environmental factors showed that some beneficial fungi and phosphorus were positively correlated,and some pathogenic fungi and potassium were negatively correlated.FUNGuild functional analysis showed that the fungi in the root and rhizosphere soil of camellia oleifera were mainly saprophytic,and the abundance of symbiotic nutritional fungi in healthy C.oleifera was the highest and gradually decreased after the disease;the phytopathogenic functional group in the root system of C.oleifera became the dominant functional group after the grade Ⅱ disease.In summary,after the disease of C.oleifera,the diversity of fungi decreasesd,the abundance of some beneficial fungi in the fibrous roots decreased,the abundance of phytopathogenic and saprophytic fungi increased,and the colonization of bush mycorrhizal fungi played an important role in regulating the disease and health relationship of C.oleifera.For the C.oleifera base in Lianghe county,Dehong prefecture,it was necessary to apply more potassium and phosphorus fertilizers and reduce the application of nitrogen fertilizers,which could increase the abundance of some beneficial fungi and reduce the abundance of pathogenic fungi to mitigate the occurrence of root rot of C.oleifera.关键词
油茶根腐病/真菌多样性/群落结构/土壤理化性质/FUNGuild功能Key words
Camellia oleifera root rot/fungal diversity/community structure/physical and chemical properties of soil/FUNGuild function分类
农业科技引用本文复制引用
杨娅琳,吴峰婧琳,陈健鑫,武自强,刘丽,张东华,马焕成,伍建榕..油茶根腐病根际土壤、根系内真菌群落结构和多样性分析[J].中国农业科技导报,2024,26(7):121-135,15.基金项目
国家重点研发计划项目(2019YFD1002002) （2019YFD1002002）
国家自然科学基金项目(31860208). （31860208）
