中国药科大学学报2024，Vol.55Issue(3)：390-396,7.DOI:10.11665/j.issn.1000-5048.2024030401
氟溴唑仑的奖赏效应及其神经环路机制研究
Reward effect of flubromazolam and its underlying neural circuit mechanism
摘要
Abstract
Flubromazolam(Flub)is a novel psychoactive substance of benzodiazepines and the mechanism underlying its addiction still remains elusive.This study investigated the reward effect of Flub using conditioned place preference(CPP)mouse model.The neuronal activity was evaluated by c-Fos expression,and the neural circuit was tracked by virus tracing.This study also investigated the regulatory effect of neural circuits on Flub-induced reward effects through chemogenetic approach.The results showed that,at the dose of 3 mg/kg,Flub significantly increased CPP score and c-Fos expression in dopaminergic(DA)neurons of ventral tegmental area(VTA).Inhibition of VTA dopaminergic neuron activity dramatically decreased Flub-induced CPP score.Virus tracing verified GABAergic neuronal projection of medial rostrum tegmental nucleus(RMTg)to VTA dopaminergic neurons.Activation of RMTgGABA→VTADA circuit or blockade of benzodiazepine receptors(BZR)in RMTg significantly decreased Flub-induced CPP score.These results indicate that Flub produced reward effect via BZR-mediated RMTgGABA→VTADA circuit.关键词
氟溴唑仑/奖赏效应/条件性位置偏好/腹侧被盖区/喙内侧被盖核/苯二氮䓬受体Key words
flubromazolam/reward effect/conditioned place preference/ventral tegmental area/rostromedial tegmental nucleus/benzodiazepine receptor分类
医药卫生引用本文复制引用
黄为国,江炜楷,邵雨微,陈志刚,唐苏苏,洪浩..氟溴唑仑的奖赏效应及其神经环路机制研究[J].中国药科大学学报,2024,55(3):390-396,7.基金项目
This study was supported by the Open Project of Key Laboratory of Drug Monitoring and Control,Ministry of Public Security(No.2021-KLDMC-04) （No.2021-KLDMC-04）
and the National Key Research and Development Program of China(No.2023YFC3304201) 公安部重点实验室开放课题(No.2021-KLDMC-04) （No.2023YFC3304201）
国家重点研发计划项目(No.2023YFC3304201) （No.2023YFC3304201）
