中国农业科技导报2024，Vol.26Issue(7)：136-146,11.DOI:10.13304/j.nykjdb.2023.0034
连作烟草根系分泌物鉴定及潜在化感物质的筛选研究
Identification of Continuous Cropping Tobacco Root Exudates and Screening of Potential Allelopathic Substances
摘要
Abstract
To explore the components of allelopathic substances contained in tobacco root exudates under long-term continuous cropping,quartz sand cultivation and continuous soil cultivation 2 tobacco culture experiments were set up,root exudates of non-continuous cropping and continuous cropping tobacco at different growth stages were extracted,and the extracted root exudates were identified and analyzed by ultra-high performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS),then potential allelopathic substances in the root exudates of continuous cropping tobacco were screened by database comparison,difference analysis and time cluster analysis.The results showed that a total of 11 potential allelopathic substances were screened,namely alkaloids,terpenes,amines,organic acids,benzic acids and derivatives,fatty acids,phenols,amino acids and peptides,coumarins,cinnamic acid and derivatives and others.The relative content of alkaloids,terpenes,amines and organic acids accounted for 73.3%of all compounds.L-nicotine,ursolic acid,β-aminopropionitrile,5-hydroxyindoleacetic acid,precocene Ⅱ,monoethylhexylphthalic acid,caprolactam,gingerol,L-lactic acid,diethyl phthalate,and methyl 2,3-dihydro-3,5-dihydroxy-2-oxo-3-indoleacetate were potential allelopathic substances with relatively high levels in continuous tobacco root exudates.This study provided a theoretical basis for the study of allelopathic substances in tobacco roots and the research on methods to alleviate tobacco continuous cropping disorders.关键词
烟草/根系分泌物/化感物质/化感作用/连作障碍/UHPLC-MS/MSKey words
tobacco/root exudates/allelopathic substances/allelopathy/continuous cropping disorder/UHPLC-MS/MS分类
农业科技引用本文复制引用
周喜新,袁世林,杨柳,夏滔,张毅,范伟..连作烟草根系分泌物鉴定及潜在化感物质的筛选研究[J].中国农业科技导报,2024,26(7):136-146,11.基金项目
湖南省烟草公司长沙市公司项目20-22A07) （）
湖南省教育厅资助科研项目(20A230) （20A230）
湖南省高新技术产业科技创新引领计划项目(2020NK2005). （2020NK2005）
