中国药科大学学报2024，Vol.55Issue(3)：397-403,7.DOI:10.11665/j.issn.1000-5048.2023032801
基于硝基化T细胞表位的PD-L1疫苗佐剂的筛选
Screening of adjuvant for PD-L1 vaccine based on nitrated T cell epitope
摘要
Abstract
To enhance the anti-tumor activity of tumor vaccine targeting PD-L1 based on the nitrated T-epitope(PD-L1-NitraTh),this research compared several adjuvants with different mechanisms to screen out the adjuvant most suitable for PD-L1-NitraTh.The results showed that Poly(I:C),CPG1018,swollen knotted polysaccharide SGP2 and GM-CSF could enhance the immunogenicity of PD-L1-NitraTh when used as adjuvants,with the Poly(I:C)group inducing the highest antibody titer.The results of qPCR for T cell differentiation-related cytokines showed that Poly(I:C)reduced the expression of GATA3 and FoxP3,indicating a strong effect on CD4+T cell differentiation.Besides,compared with other adjuvants,Poly(I:C)could assist PD-L1-NitraTh to increase the infiltration of T cells as well as CD11b+cells within tumor,suggesting that Poly(I:C)may be the suitable adjuvant for tumor vaccines based on the nitrated T epitopes.关键词
肿瘤疫苗/佐剂/Toll样受体/Poly(I:C)Key words
tumor vaccine/adjuvant/Toll-like receptor/Poly(I:C)分类
医药卫生引用本文复制引用
周虹佑,高向东,姚文兵,田浤..基于硝基化T细胞表位的PD-L1疫苗佐剂的筛选[J].中国药科大学学报,2024,55(3):397-403,7.基金项目
This study was supported by the National Natural Science Foundation of China(No.81973222,No.82073754)and the Key Research and Development Program of Xinjiang Uygur Autonomous Region(No.2020B03003) 国家自然科学基金项目(No.81973222,No.82073754) （No.81973222,No.82073754）
新疆维吾尔自治区重点研发计划项目(No.2020B03003) （No.2020B03003）
