鸡骨草水提物灌胃大鼠后的体内成分及代谢途径分析OA北大核心CSTPCD

OBJECTIVE To analyze the chemical components that were the absorbed in blood and liver tissue of rats after intragastric administration of aqueous extract from Abrus cantoniensis,and to speculate its possible metabolic pathways,providing reference for basic analysis of pharmacological substance in A.cantoniensis.METHODS Male SD rats were randomly divided into A.cantoniensis group(0.63 g/kg,calculated by crude drug)and blank group;they were given relevant drug solution/ultrapure water intragastrically.After a single dose,plasma and liver samples of rats in each group were collected.UPLC-Q-TOF/MS technology was used to identify chemical components that were absorbed in the blood and liver tissue of rats.RESULTS Totally,30 chemical constituents were identified from the water extracts of A.cantoniensis,including alkaloids,flavonoids,organic acids,iridoids(such as L-abrine,schaftoside,isoshaftoside).Ten prototype components and nine metabolites(such as decarboxylation and sulfation metabolites of protocatechuic acid,reduced sulfated metabolites of p-hydroxybenzoic acid)were identified from plasma samples;six prototype components and five metabolites(such as sulfated metabolites of p-hydroxybenzoic acid,decarboxylation and sulfation metabolites of p-hydroxybenzoic acid)were identified from liver samples.The main metabolic pathways included hydroxylation,demethylation,methylation,sulfation,glucuronidation,etc.CONCLUSIONS Alkaloids,flavonoids and organic acids are the main components of the aqueous extract from A.cantoniensis that are absorbed into the blood and liver,their metabolism mainly involves hydroxylation,demethylation,and sulfation.