中国药房2024，Vol.35Issue(13)：1576-1581,6.DOI:10.6039/j.issn.1001-0408.2024.13.06
鸡骨草水提物灌胃大鼠后的体内成分及代谢途径分析
Analysis of in vivo components and metabolism pathway in rats after intragastric administration of aqueous extract from Abrus cantoniensis
摘要
Abstract
OBJECTIVE To analyze the chemical components that were the absorbed in blood and liver tissue of rats after intragastric administration of aqueous extract from Abrus cantoniensis,and to speculate its possible metabolic pathways,providing reference for basic analysis of pharmacological substance in A.cantoniensis.METHODS Male SD rats were randomly divided into A.cantoniensis group(0.63 g/kg,calculated by crude drug)and blank group;they were given relevant drug solution/ultrapure water intragastrically.After a single dose,plasma and liver samples of rats in each group were collected.UPLC-Q-TOF/MS technology was used to identify chemical components that were absorbed in the blood and liver tissue of rats.RESULTS Totally,30 chemical constituents were identified from the water extracts of A.cantoniensis,including alkaloids,flavonoids,organic acids,iridoids(such as L-abrine,schaftoside,isoshaftoside).Ten prototype components and nine metabolites(such as decarboxylation and sulfation metabolites of protocatechuic acid,reduced sulfated metabolites of p-hydroxybenzoic acid)were identified from plasma samples;six prototype components and five metabolites(such as sulfated metabolites of p-hydroxybenzoic acid,decarboxylation and sulfation metabolites of p-hydroxybenzoic acid)were identified from liver samples.The main metabolic pathways included hydroxylation,demethylation,methylation,sulfation,glucuronidation,etc.CONCLUSIONS Alkaloids,flavonoids and organic acids are the main components of the aqueous extract from A.cantoniensis that are absorbed into the blood and liver,their metabolism mainly involves hydroxylation,demethylation,and sulfation.关键词
鸡骨草/血浆/肝脏/UPLC-Q-TOF/MS/药源性成分Key words
Abrus cantoniensis/plasma/liver/UPLC-Q-TOF/MS/drug-derived ingredients分类
医药卫生引用本文复制引用
陈奎奎,农雨欣,郭忠会,谭勇,黄宏婷,黎金满,韦金锐,韦志英,梁洁..鸡骨草水提物灌胃大鼠后的体内成分及代谢途径分析[J].中国药房,2024,35(13):1576-1581,6.基金项目
国家自然科学基金区域创新发展联合基金重点支持项目(No.U23A20521) （No.U23A20521）
国家自然科学基金项目(No.82160771) （No.82160771）
广西科技基地与人才专项(No.桂科AD21238032) （No.桂科AD21238032）
广西自然科学基金项目(No.2022JJB140286) （No.2022JJB140286）
广西中医药大学第三批"岐黄工程"高层次人才团队培育项目(No.202406) （No.202406）
广西中医药大学青年创新团队项目(No.2018QT001) （No.2018QT001）
广西中医药大学博士科研启动基金项目(No.2020BS015) （No.2020BS015）
广西中医药大学青年基金项目(No.2021QN006) （No.2021QN006）
