中国药房2024，Vol.35Issue(13)：1588-1593,6.DOI:10.6039/j.issn.1001-0408.2024.13.08
麻黄与杏仁配伍后在支气管哮喘模型大鼠体内的药动学研究
Pharmacokinetic study of the complication of Ephedra sinica and Prunus armeniaca in bronchial asthma model rats
摘要
Abstract
OBJECTIVE To study the pharmacokinetic changes in the plasma and cerebrospinal fluid of bronchial asthma model rats after the complication of Ephedra sinica and Prunus armeniaca.METHODS SD male rats were randomly divided into blank group,model group,E.sinica group(12 g/kg,calculated by raw drug,similarly hereinafter),P.armeniaca group(6 g/kg)and E.sinica-P.armeniaca drug-pair group(12 g/kg of E.sinica+6 g/kg of P.armeniaca),with 6 rats in each group.Except for the blank group,the bronchial asthma model was induced by spraying rats in each group with an equal volume mixture of 2%acetylcholine chloride and 0.4%histamine phosphate,once a day,for 7 d.One hour before modeling every time,rats in each group were gavaged with the corresponding drug/normal saline,once a day,for 7 d.After the final administration and provocation of asthma,blood and cerebrospinal fluid collection were performed at different time points.The plasma and cerebrospinal fluid samples were pre-treated(with geranylgeranyl as the internal standard),and the mass concentrations of ephedrine/pseudoephedrine,methyl ephedrine and amygdalin in both samples were determined by liquid chromatography-tandem mass spectrometry.DAS 2.0 pharmacokinetic software was used to determine the main pharmacokinetic parameters through the non-atrial chamber model and to compare the changes of the pharmacokinetic parameters before and after the combination of the two drugs.RESULTS Compared with E.sinica group,cmax and AUC0-21.33 h(or AUC0-10.67 h)of ephedrine/pseudoephedrine and methyl ephedrine in the plasma and cerebrospinal fluid of rats were significantly reduced in E.sinica-P.armeniaca drug-pair group,while CLZ/F and VZ/F were significantly increased(P＜0.05 or P＜0.01);tmax of methyl ephedrine in the cerebrospinal fluid was significantly shortened(P＜0.05).Compared with P.armeniaca group,the t1/2 of amygdalin in the plasma of rats in E.sinica-P.armeniaca drug-pair group was significantly shortened,and CLZ/F was significantly increased(P＜0.01);the tmax of bitter amygdalin in the cerebrospinal fluid was significantly shortened,and the AUC0-10.67 h,CLZ/F,and VZ/F were significantly increased(P＜0.01).CONCLUSIONS The combination of E.sinica and P.armeniaca accelerates the absorption and elimination of ephedra alkaloids,thus reducing the accumulation of ephedra alkaloids in the bronchial asthma model rats.关键词
麻黄/杏仁/配伍/哮喘/药动学Key words
Ephedra sinica/Prunus armeniaca/complica-tion/asthma/pharmacokinetics分类
医药卫生引用本文复制引用
陈林林,谢金根,樊学程,饶倩,杨添翼,田佳玉,肖雄,高文军,李文宏..麻黄与杏仁配伍后在支气管哮喘模型大鼠体内的药动学研究[J].中国药房,2024,35(13):1588-1593,6.基金项目
国家自然科学基金项目(No.82060790,No.82360856) （No.82060790,No.82360856）
江西省自然科学基金资助项目(No.20202ACBL206031) （No.20202ACBL206031）
