外周血T淋巴细胞亚群、NK细胞、B细胞及IL-6在不同分期慢性肾脏病患者中的表达及临床意义OA
Expression and Clinical Significance of T Lymphocyte Subsets,NK Cell,B Cell and IL-6 in Peripheral Blood in Patients with Different Stages of Chronic Kidney Disease
目的:探讨外周血T淋巴细胞亚群、自然杀伤细胞(NK细胞)、B细胞及白细胞介素-6(IL-6)在不同分期慢性肾脏病(chronic kidney disease,CKD)患者中的表达及临床意义.方法:选取盐城市中医院肾内科 2022 年 10 月—2023 年 6 月收治的 80 例CKD 3～5 期患者设为CKD组,另外选择同期 21 例健康体检者设为对照组,其中CKD组患者根据肾小球滤过率水平分为CKD 3 期组、CKD 4 期组、CKD 5 期组.用流式细胞术检测外周血T淋巴细胞亚群(CD4+T细胞、CD8+T细胞)、NK细胞、B细胞,用酶联免疫吸附试验检测IL-6 水平.比较以上指标在不同分期CKD患者中的表达,并采用Spearman相关性分析以上指标与CKD严重程度的相关性.结果:CKD 3 期组、CKD 4 期组、CKD 5 期组CD4+T细胞、CD8+T细胞、NK细胞、B细胞均明显低于对照组,且CD4+T细胞、CD8+T细胞、NK细胞、B细胞随CKD严重程度加重逐渐降低,差异均有统计学意义(P<0.05);CKD 3 期组、CKD 4 期组、CKD 5 期组IL-6 水平明显高于对照组,且IL-6 水平随CKD严重程度加重逐渐升高,差异均有统计学意义(P<0.05);IL-6 水平与CKD分期呈负相关,CD4+T细胞、CD8+T细胞、NK细胞、B细胞与CKD分期呈正相关(P<0.05).结论:随着CKD进展,患者的免疫功能受到显著影响,外周血T淋巴细胞亚群、NK细胞、B细胞及IL-6 在评估CKD患者疾病进展中有重要临床意义.
Objective:To investigate the expression and clinical significance of T lymphocyte subsets,natural killer cell(NK cell),B cell and interleukin-6(IL-6)in peripheral blood in patients with different stages of chronic kidney disease(CKD).Method:A total of 80 patients with CKD stage 3-5 admitted to the Department of Nephrology,Yancheng Traditional Chinese Medicine Hospital from October 2022 to June 2023 were selected as the CKD group,and 21 healthy subjects in the same period were selected as the control group.Patients in the CKD group were divided into CKD stage 3 group,CKD stage 4 group and CKD stage 5 group according to the glomerular filtration rate level.T lymphocyte subsets(CD4+T cell,CD8+T cell),NK cell and B cell in peripheral blood were detected by flow cytometry,and IL-6 levels were detected by enzyme linked immunosorbent assay.The expression of the above indexes in patients with different stages of CKD were compared,and Spearman correlation was used to analyze the correlation between the above indexes and the severity of CKD.Result:CD4+T cell,CD8+T cell,NK cell and B cell in CKD stage 3 group,CKD stage 4 group,CKD stage 5 group were significantly lower than those in control group,and CD4+T cell,CD8+T cell,NK cell and B cell gradually decreased with the severity of CKD,the differences were statistically significant(P<0.05).The levels of IL-6 in CKD stage 3 group,CKD stage 4 group,CKD stage 5 group were significantly higher than that in control group,and the levels of IL-6 increased gradually with the severity of CKD,the differences were statistically significant(P<0.05).IL-6 level was negatively correlated with CKD stage,and CD4+T cell,CD8+T cell,NK cell and B cell were positively correlated with CKD stage(P<0.05).Conclusion:With the progression of CKD,the immune function of patients is significantly affected,and peripheral blood T lymphocyte subsets,NK cell,B cell and IL-6 have important clinical significance in evaluating the disease progression of patients with CKD.
时洪娟;孟宪杰;秦松林
盐城市中医院肾内科 江苏 盐城 224001
慢性肾脏病T淋巴细胞亚群白细胞介素-6
Chronic kidney diseaseT lymphocyte subsetsInterleukin-6
《中国医学创新》 2024 (016)
99-102 / 4
盐城市医学科技发展计划项目(YK2021040)
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