中华骨质疏松和骨矿盐疾病杂志2024，Vol.17Issue(3)：221-229,9.DOI:10.3969/j.issn.1674-2591.2024.03.004
基于绝经后女性股骨样本的骨髓造血干细胞自噬与骨质疏松相关性研究
Correlation between autophagy of bone marrow hematopoietic stem cells and osteoporosis in femoral bone samples of postmenopausal women
摘要
Abstract
Objective To invetigate the correlation between autophagy of hematopoietic stem cells and osteopo-rosis in femoral bone samples of postmenopausal women obtained from hip fracture surgery.Methods Bone marrow of 19 postmenopausal women were collected from hip replacement surgery and the patients were divided into osteoporosis group(n=13)and non-osteoporosis group(n=6)according to the bone mineral density at femoral neck and lumbar spine.Hematopoietic stem cells were obtained by flow cytometry.Hematopoietic stem cells autophagy was measured by imaging flow cytometry.Reverse transcription PCR(RT-PCR)was performed to determine the expressions of autophagy related genes,including Atg7,LC3b,LAMP2a,P62,Atg5,Atg12.Ovariectomy osteoporosis mice model were established for collecting bone marrow hematopoietic stem cells.RT-PCR was performed to determine the expressions of autophagy related genes,including Atg7,LC3b,LAMP2a,P62,Atg5,Atg12.Autophagy related proteins were measured by western blot in mice.The expression of vascular endothelial growth factor A(VEGF-A)was detected when abnormal autophagy of bone marrow hematopoietic stem cells.Results Hematopoietic stem cells autophagy was inhibited in patients with osteoporosis and the expressions of autophagy related genes including Atg7,LC3b,LAMP2a,P62,Atg5,Atg12 were down-regulated(P＜0.05).The expressions of ATG7,BECLIN1 and LC3-Ⅰ/LC3-Ⅱ were inhibited in osteoporotic mice.The expres-sions of autophagy related genes including Atg7,LC3b,LAMP2a,P62,Atg5,Atg12 were inhibited in ovariectomy osteo-porosis mice and the expression of VEGF-A was reduced after the inhibition of the autophagy(P＜0.05).Conclusion Inhibition of hematopoietic stem cells autophagy is related to osteoporosis.关键词
髋部骨折/造血干细胞/骨质疏松/自噬Key words
hip fracture/hematopoietic stem cells/osteoporosis/autophagy分类
医药卫生引用本文复制引用
袁晔,王晓娟,朱柯雨,魏祺,郑苗,邢丹,徐又佳,林剑浩..基于绝经后女性股骨样本的骨髓造血干细胞自噬与骨质疏松相关性研究[J].中华骨质疏松和骨矿盐疾病杂志,2024,17(3):221-229,9.基金项目
国家重点研发计划资助(2021YFC2501700、2021YFC2501702) （2021YFC2501700、2021YFC2501702）
国家自然科学基金(82372455、82272538) （82372455、82272538）
北京市自然科学基金-海淀原始创新联合基金(L222087) （L222087）
北京大学人民医院研究与发展基金资助项目(RDJP2023-21) （RDJP2023-21）
江苏省医学重点实验室建设单位(JSDW202254) （JSDW202254）
