基于绝经后女性股骨样本的骨髓造血干细胞自噬与骨质疏松相关性研究OA北大核心CSTPCD

Objective To invetigate the correlation between autophagy of hematopoietic stem cells and osteopo-rosis in femoral bone samples of postmenopausal women obtained from hip fracture surgery.Methods Bone marrow of 19 postmenopausal women were collected from hip replacement surgery and the patients were divided into osteoporosis group(n=13)and non-osteoporosis group(n=6)according to the bone mineral density at femoral neck and lumbar spine.Hematopoietic stem cells were obtained by flow cytometry.Hematopoietic stem cells autophagy was measured by imaging flow cytometry.Reverse transcription PCR(RT-PCR)was performed to determine the expressions of autophagy related genes,including Atg7,LC3b,LAMP2a,P62,Atg5,Atg12.Ovariectomy osteoporosis mice model were established for collecting bone marrow hematopoietic stem cells.RT-PCR was performed to determine the expressions of autophagy related genes,including Atg7,LC3b,LAMP2a,P62,Atg5,Atg12.Autophagy related proteins were measured by western blot in mice.The expression of vascular endothelial growth factor A(VEGF-A)was detected when abnormal autophagy of bone marrow hematopoietic stem cells.Results Hematopoietic stem cells autophagy was inhibited in patients with osteoporosis and the expressions of autophagy related genes including Atg7,LC3b,LAMP2a,P62,Atg5,Atg12 were down-regulated(P＜0.05).The expressions of ATG7,BECLIN1 and LC3-Ⅰ/LC3-Ⅱ were inhibited in osteoporotic mice.The expres-sions of autophagy related genes including Atg7,LC3b,LAMP2a,P62,Atg5,Atg12 were inhibited in ovariectomy osteo-porosis mice and the expression of VEGF-A was reduced after the inhibition of the autophagy(P＜0.05).Conclusion Inhibition of hematopoietic stem cells autophagy is related to osteoporosis.