中国中医药信息杂志2024，Vol.31Issue(7)：100-105,6.DOI:10.19879/j.cnki.1005-5304.202312092
推拿对坐骨神经慢性压迫损伤大鼠脊髓背角小胶质细胞P2Y12/RhoA/ROCK2通路及c-Fos蛋白表达的影响
Effects of Tuina on P2Y12/RhoA/ROCK2 Pathway and c-Fos Protein Expression of Microglia in Spinal Dorsal Horn of Sciatic Nerve Chronic Compressive Injury Rats
摘要
Abstract
Objective To observe the effects of tuina on P2Y12/RhoA/ROCK2 pathway and c-Fos protein expression of microglia in spinal dorsal horn of sciatic nerve chronic compressive injury(CCI)rats;To explore the mechanism of tuina in treating lumbar disc herniation.Methods A CCI model of right sciatic nerve was used to simulate neuropathic pain in lumbar disc herniation.24 male SD rats were randomly divided into blank group,model group and tuina group,with 8 rats in each group.After 4 days of modeling,massage intervention was used to in the tuina group for 14 days.The paw withdrawal threshold(PWT)and paw withdrawal latency(PWL)of rats before and on the 4th,10th,and 17th day after modeling were observed,immunofluorescence staining was used to detect the expression of Iba1 and P2Y12 protein in right spinal dorsal horn,Western blot was used to detect the expressions of RhoA and ROCK2 protein in right spinal dorsal horn,immunohistochemistry staining was used to detect the number of c-Fos positive cells in right spinal dorsal horn.Results Compared with the blank group,the PWT and PWL of the model group were significantly reduced on the 4th,10th,and 17th day after modeling(P<0.001),while the expressions of Iba1,P2Y12,RhoA and ROCK2 protein in right spinal dorsal horn significantly increased(P<0.001,P<0.05),the number of c-Fos positive cells significantly increased(P<0.001).Compared with the model group,the PWT and PWL of the tuina group significantly increased on the 10th and 17th day after modeling(P<0.05,P<0.01,P<0.001),and the expressions of Iba1,P2Y12,RhoA and ROCK2 protein in right spinal dorsal horn significantly decreased(P<0.001,P<0.01,P<0.05),and the number of c-Fos positive cells significantly reduced(P<0.001).Conclusion Tuina may inhibit the activation of microglia by regulating P2Y12/RhoA/ROCK2 pathway and c-Fos protein expression in spinal dorsal horn,reduce neuronal excitability,and exert analgesic effects on lumbar disc herniation.关键词
腰椎间盘突出症/推拿/脊髓背角/小胶质细胞/P2Y12/RhoA/ROCK2通路/c-Fos/大鼠Key words
lumbar disc herniation/tuina/spinal dorsal horn/microglia/P2Y12/RhoA/ROCK2 pathway/c-Fos/rats分类
医药卫生引用本文复制引用
蒋晶晶,林志刚,黄红叶,张幻真,陈乐春,林惠,伍诗烨,陈水金..推拿对坐骨神经慢性压迫损伤大鼠脊髓背角小胶质细胞P2Y12/RhoA/ROCK2通路及c-Fos蛋白表达的影响[J].中国中医药信息杂志,2024,31(7):100-105,6.基金项目
国家自然科学基金青年科学基金(82205303、82105039) （82205303、82105039）
国家自然科学基金面上项目(82174523) （82174523）
福建省自然科学基金面上项目(2023J01878、2020J01760) （2023J01878、2020J01760）
福建省卫健委医学创新课题(2020CXA052) （2020CXA052）
福建省卫健委中青年骨干人才培养项目(2021GGA060) （2021GGA060）
