中国癌症防治杂志2024，Vol.16Issue(3)：271-276,6.DOI:10.3969/j.issn.1674-5671.2024.03.02
Omicron变异株XBB.1.16刺突蛋白对人非小细胞肺癌细胞的生物学功能的影响
Effects of Omicron variant XBB.1.16 spike protein on biological function of human non-small cell lung cancer cells
摘要
Abstract
Objective To investigate the virological characteristics of human non-small cell lung cancer cell line H1299-ACE2 infected with Omicron variant XBB.1.16 spike protein(XBB.1.16-S)and its effect on mitochondrial damage in H1299-ACE2 cells.Methods The XBB.1.16-S gene eukaryotic expression vector(pCMV3-XBB.1.16-S plasmid)was constructed by using the parental pCMV3-SARS-CoV-2-S plasmid as a template and transfected into H1299-ACE2 cells,the ability of inducing syncytial formation was compared.The H1299-ACE2 cells were infected with SARS-CoV-2-S and XBB.1.16-S pseudoviruses after packaging with a double plasmid system,respectively.The luciferase intensity of the host cells after infection was determined.The cleavage efficiency of S protein in the cells was detected by Western blot.MitoTracker green probe was used to observe mitochondrial morphology of stained cells.The levels of mitochondrial membrane potential(MMP)and reactive oxygen species(ROS)of the cells were detected by flow cytometry.Results Compared with the parental pCMV3-SARS-CoV-2-S plasmid,key amino acid mutation sites such as T19I,V83A,and G142D in the pCMV3-XBB.1.16-S plasmid were successfully mutated,and the area of the syncytium formed after transfection into H1299-ACE2 cells was significantly reduced(P＜0.0001).Compared with those of SARS-CoV-2-S,the luciferase intensity,S protein cleavage efficiency and ROS level of H1299-ACE2 cells after being infected with XBB.1.16-S were decreased(all P＜0.05),while mitochondrial length was longer(P＜0.0001),the MMP level was increased(P＜0.001).Conclusions The infectivity and mitochondrial damage ability of Omicron variant XBB.1.16-S on non-small cell lung cancer H1299-ACE2 cells were weakened to some extent compared with the parental virus.This finding lays an im-portant foundation for the study of cellular changes and treatment targets in lung cancer complicated by COVID-19.关键词
非小细胞肺癌/Omicron/XBB.1.16/H1299-ACE2细胞/线粒体Key words
Non-small cell lung cancer/Omicron/XBB.1.16/H1299-ACE2 cells/Mitochondrion分类
医药卫生引用本文复制引用
房廖鑫,亢小峰,薛春源,潘露,陈佳欣,汤传昊,孙丽颖,徐小洁,杜祎萌..Omicron变异株XBB.1.16刺突蛋白对人非小细胞肺癌细胞的生物学功能的影响[J].中国癌症防治杂志,2024,16(3):271-276,6.基金项目
国家自然科学基金项目(82202852) （82202852）
