中国药业2024，Vol.33Issue(13)：56-58,3.DOI:10.3969/j.issn.1006-4931.2024.13.013
高效液相色谱法测定人血浆中伏立康唑浓度
Determination of Concentration of Voriconazole in Human Plasma by HPLC
摘要
Abstract
Objective To establish a high-performance liquid chromatography(HPLC)method for the determination of the concentration of voriconazole in human plasma.Methods Acetonitrile protein precipitation method was used to process blood samples.The chromatographic column was InsertSustain C18 column(250 mm×4.6 mm,5 μm),the mobile phase was methanol-acetonitrile-phosphate buffer(33∶28∶39,V/V/V),the flow rate was 1.0 mL/min,the column temperature was 30℃,the detection wavelength was 262 nm,and the injection volume was 20 μL.Results The linear range of voriconazole was 0.1-8 μg/mL(R2=0.999 8,n=8).The limit of quantification was 0.07 μg/mL.The RSDs of the intra-day and inter-day precision,freeze-thaw,and long-term stability test results for samples with low-,medium-,and high-concentrations of 0.125,1,8 μg/mL were all lower than 5% .The extraction recovery rates were(97.32±1.71)%,(97.61±1.22)%,and(96.38±0.84)%,respectively.In the plasma of 60 patients diagnosed with invasive fungal infections,the concentration of voriconazole was in the range of 0.278-9.129 μg/mL.Conclusion The established method is simple,rapid,and has good specificity,which is suitable for monitoring the blood concentration of voriconazole.关键词
伏立康唑/高效液相色谱法/血药浓度Key words
voriconazole/HPLC/blood concentration分类
医药卫生引用本文复制引用
朱丽莎,苏强,唐春梅..高效液相色谱法测定人血浆中伏立康唑浓度[J].中国药业,2024,33(13):56-58,3.基金项目
四川省南充市科学技术和知识产权局首批重点实验室建设项目[NCKL201711] （）
川北医学院校级科研发展计划项目[CBY17-A-YB55]. （）
