中国造纸学报2024，Vol.39Issue(2)：44-51,8.DOI:10.11981/j.issn.1000-6842.2024.02.44
漆酶介体系统催化碱木质素降解及其结构演变研究
Catalytic Degradation of Alkali Lignin by Laccase Mediator System and Its Structural Evolution Studies
摘要
Abstract
Laccase-mediator system(LMS),which uses the mediator as an electron shuttle,has been widely used in the degradation of envi-ronmental pollutants because of its higher catalytic efficiency for substrates.This study systematically investigated the degradation effects of laccase and laccase/H2O2,laccase/HBT(1-hydroxybenzotriazole)and laccase/ABTS(2,2'-bisazobis(3-ethylbenzothiazolin-6-sulfonic acid)diammonium salt)on alkali lignin,and explored the influencing factors of laccase/ABTS mediator system on degradation of alkali lignin.The structural characterization of alkali lignin before and after the reaction with laccase and the three LMS was also analyzed.The results showed that the highest degradation rate of alkali lignin was achieved by the laccase/ABTS mediator system,which could reach 39%under the reac-tion conditions at 32℃for 24 h with pH valve of 5,and ABTS concentration of 2 mmol/L.The types of functional groups of alkali lignin ob-tained from the laccase treatment remained unchanged,but the polydispersity coefficient was decreased and the molecular mass was in-creased;and the polydispersity coefficient and the molecular mass of alkali lignin obtained from the LMS treatment were further decreased,especially the polydispersity coefficient and the molecular mass of alkali lignin obtained from the LMS treatment.In particular,the number average molecular weight of alkali lignin was reduced by 50.5%after laccase/ABTS treatment;the chemical bonds of alkali lignin were broken to different degrees after LMS treatment,which induced changes such as C—O and β-O-4 linkage breaking,demethylation reaction,and ben-zene ring-opening.关键词
碱木质素/漆酶/介体/降解Key words
alkali lignin/laccase/mediator/degradation分类
轻工纺织引用本文复制引用
崔丁凯,易国斌,叶大威,朱国志,肖盈,陈佳志..漆酶介体系统催化碱木质素降解及其结构演变研究[J].中国造纸学报,2024,39(2):44-51,8.基金项目
广东省基础与应用基础研究基金(2022A1515012584,2022A1515110640) （2022A1515012584,2022A1515110640）
广东省科学院专项资金(2022GDASZH-2022010105). （2022GDASZH-2022010105）
