高效液相色谱法测定复方黄番口服液中芍药苷含量OACSTPCD
Content Determination of Paeoniflorin in Compound Huangfan Oral Liquid by HPLC
目的 建立测定复方黄番口服液中芍药苷含量的高效液相色谱法.方法 色谱柱为Agilent Eclipse Plus C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸溶液(26∶74,V/V),流速为 1.0 mL/min,检测波长为 230 nm,柱温为 30℃,进样量为 10 μL.结果 芍药苷的质量浓度在 15.35～168.85 μg/mL范围内与峰面积线性关系良好(r=0.999 6,n=6);精密度、稳定性、重复性试验结果的RSD均小于 1.0%(n=6);平均回收率为 95.03%,RSD为 0.78%(n=9).结论 该方法结果准确、稳定性和重复性均良好,可用于复方黄香口服液中芍药苷的含量测定.
Objective To establish a high-performance liquid chromatography(HPLC)method for the content determination of paeoniflorinof in Compound Huangfan Oral Liquid.Methods The chromatography column was Agilent Eclipse Plus C18 column(150 mm×4 6 mm,5 μm),the mobile phase was methanol-0.1% phosphoric acid solution(26∶74,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 230 nm,the column temperature was 30℃,and the injection volume was 10 μL.Results The linear range of paeoniflorin was 15.35-168.85 μg/mL(r=0.999 6,n=6).The RSDs of precision,stability,and repeatability test results were all lower than 1.0% (n=6).The average recovery of paeoniflorin was 95.03% with an RSD of 0.78% (n=9).Conclusion The method is accurate,stable,and has good repeatability,which can be used for the content determination of paeoniflorin in Compound Huangfan Oral Liquid.
张育勤;米婷婷;赵俊红;辜燕茹;王曦叶
中国人民解放军西部战区总医院,四川 成都 610083川北医学院药学院,四川 南充 637000
药学
高效液相色谱法复方黄番口服液芍药苷含量测定
HPLCCompound Huangfan Oral Liquidpaeoniflorincontent determination
《中国药业》 2024 (013)
74-76 / 3
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