中国药业2024，Vol.33Issue(13)：74-76,3.DOI:10.3969/j.issn.1006-4931.2024.13.017
高效液相色谱法测定复方黄番口服液中芍药苷含量
Content Determination of Paeoniflorin in Compound Huangfan Oral Liquid by HPLC
张育勤 1米婷婷 1赵俊红 1辜燕茹 2王曦叶1
作者信息
- 1. 中国人民解放军西部战区总医院,四川 成都 610083
- 2. 川北医学院药学院,四川 南充 637000
- 折叠
摘要
Abstract
Objective To establish a high-performance liquid chromatography(HPLC)method for the content determination of paeoniflorinof in Compound Huangfan Oral Liquid.Methods The chromatography column was Agilent Eclipse Plus C18 column(150 mm×4 6 mm,5 μm),the mobile phase was methanol-0.1% phosphoric acid solution(26∶74,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 230 nm,the column temperature was 30℃,and the injection volume was 10 μL.Results The linear range of paeoniflorin was 15.35-168.85 μg/mL(r=0.999 6,n=6).The RSDs of precision,stability,and repeatability test results were all lower than 1.0% (n=6).The average recovery of paeoniflorin was 95.03% with an RSD of 0.78% (n=9).Conclusion The method is accurate,stable,and has good repeatability,which can be used for the content determination of paeoniflorin in Compound Huangfan Oral Liquid.关键词
高效液相色谱法/复方黄番口服液/芍药苷/含量测定Key words
HPLC/Compound Huangfan Oral Liquid/paeoniflorin/content determination分类
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张育勤,米婷婷,赵俊红,辜燕茹,王曦叶..高效液相色谱法测定复方黄番口服液中芍药苷含量[J].中国药业,2024,33(13):74-76,3.
