中国药业2024，Vol.33Issue(13)：77-80,4.DOI:10.3969/j.issn.1006-4931.2024.13.018
淫芎颗粒质量标准研究
Quality Standard of Yinxiong Granules
摘要
Abstract
Objective To establish the quality standard of Yixiong Granules.Methods The thin-layer chromatography(TLC)method was used for the qualitative identification of Chuanxiong Rhizoma and Paeoniae Radix Alba in the preparation.The high-performance liquid chromatography(HPLC)method was used for the content determination of paeoniflorin,liquiritin,ferulic acid,naringin,hesperidin,icariin,and ammonium glycyrrhizinate in the preparation.The chromatographic column was Agilent Zorbax SB-C18 column(250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.3% phosphoric acid solution with gradient elution,the flow rate was 1.0 mL/min,the detection wavelength was 230 nm,the column temperature was 30℃,and the injection volume was 10 μL.Results TLC spots were clear,with good separation,and the negative control had no interference.The linear ranges of paeoniflorin,liquiritin,ferulic acid,naringin,hesperidin,icariin,and ammonium glycyrrhizinate were 8.886-222.156 μg/mL,3.935-98.385 μg/mL,3.805-95.118 μg/mL,4.457-111.416 μg/mL,4.574-114.360 μg/mL,3.512-87.794 μg/mL,and 4.274-106.850 μg/mL(r≥0.999 1,n=7),respectively.The RSDs of precision,stability,and repeatability test results were all lower than 3.0% .The average recoveries of the above seven components were 99.68%,99.19%,100.31%,100.70%,99.74%,98.77%,and 98.99% y,with RSDs of 1.57%,1.89%,2.77%,1.52%,1.58%,1.27%,and 2.18% (n=9),respectively.The average contents of in samples were 5.743,0.547,0.472,1.426,1.376,1.982,and 1.480 μg/mL(n=2),respectively.Conclusion The method is simple,accurate,with good specificity and repeatability,which can be used for the quality control of Yinxiong Granules.关键词
淫芎颗粒/薄层色谱法/高效液相色谱法/定性鉴别/含量测定Key words
Yinxiong Granules/TLC/HPLC/qualitative identification/content determination分类
医药卫生引用本文复制引用
曹丽萍,林晓燕,梅全喜,冯晓婷,李佳瑞,梁奇..淫芎颗粒质量标准研究[J].中国药业,2024,33(13):77-80,4.基金项目
广东省深圳市中药特色技术传承人才培训项目 （）
广东省深圳市医疗卫生三名工程[SZZYSM202206005]. （）
