结肠癌细胞膜仿生铜基金属有机框架对DC的激活和促血管新生作用OA北大核心CSTPCD

Objective:To explore the potential of colon cancer cell membrane-coated copper-based metal-organic frameworks(MOF@CCM)in anti-tumor activities and promotion of tissue healing.Methods:Copper(Ⅱ)1,3,5-benzenetricarboxylate copper-based metal-organic framework(HKUST-1)was constructed,and coated with colon cancer cell CT26 membrane on its outer layer to obtain MOF@CCM,which was physically characterized.The biocompatibility of MOF@CCM was determined by CCK-8 assay,and the effect of MOF@CCM on the proportion of DC2.4 maturation was analyzed by flow cytometry to verify the potential of MOF@CCM in activating immune cells.Angiogenesis experiments was used to verify the potential of MOF@CCM in promoting HUVEC to form blood vessels.Results:MOF@CCM was successfully synthesized.Transmission electron microscopy showed that its shape was approximately circular and had an obvious core-shell structure.The average particle size of MOF@CCM was(150.5±7.89)nm,and the average Zeta potential was-(5.12±1.67)mV.The results of in vitro experiments showed that compared with the control group,MOF@CCM could significantly increase the proportion of DC2.4 maturation(P<0.01).In addition,both MOF and MOF@CCM could promote the formation of tubular structures in HUVEC cells(P<0.05 or P<0.01),and cell membrane modification had no significant effect on the pro-neovascularization effect of MOF.Conclusion:The synthesized MOF@CCM has good cell compatibility at the dose used and can significantly promote the maturation of DC2.4 cells and HUVEC angiogenesis.It is expected to become a bifunctional treatment platform for anti-colon cancer treatment and tissue repair promotion.