中国肿瘤生物治疗杂志2024，Vol.31Issue(6)：566-572,7.DOI:10.3872/j.issn.1007-385x.2024.06.004
连翘苷通过激活Hippo-YAP信号通路抑制结肠癌LS180细胞的恶性生物学行为
Phillyrin inhibits the malignant biolgical behaviors of colon cancer LS180 cells through activation of the Hippo/YAP signaling pathway
摘要
Abstract
Objective:To investigate the effects of phillyrin(Phi)on the proliferation,migration and invasion of colorectal cancer LS180 cells by regulating the Hippo/YAP signaling pathway.Methods:Human colon cancer LS180 cells were treated with Phi at different concentrations(0,5,10,20,40,80 µmol/L),and cell vitality at 24,48,and 72 h was detected by MTT assay.LS180 cells were divided into the Control group,the Phi-L(5 µmol/L Phi)group,the Phi-M(10 µmol/L Phi)group,the Phi-H(20 µmol/L Phi)group,and the Phi-H+YAP inhibitor verteporfin(VP)group(20 µmol/L Phi+5 µmol/L VP).EdU was applied to detect the effects of Phi on cell proliferation in each group;scratch healing test and Transwell chamber experiments were applied to detect the effects of Phi on cell migration and invasion,respectively;the effects of Phi on Ki-67 expression rate and LATS1,YAP,p-YAP,MMP-2,MMP-9,E-cadherin and N-cadherin expressions were detected by immunofluorescence and WB.A nude mouse model of LS180 cell transplanted tumor was constructed to observe the effects of Phi on the volume and mass of transplanted tumors.Ki-67 expression rate and the expression levels of LATS1,YAP and p-YAP proteins in transplanted tumor tissues were detected by immunofluorescence and WB methods.Results:Compared with the Control group,the cell EdU positive rate,scratch healing rate,cell invasion number,Ki-67 positive rate,MMP-2,MMP-9 and N-cadherin expression levels of LS180 cells in the Phi-L,Phi-M,and Phi-H groups decreased significantly(all P<0.05),while the expression levels of E-cadherin,LATS1 and p-YAP/YAP increased significantly(all P<0.05).The inhibition of Phi on the proliferation,migration and invasion of LS180 cells was partially reversed when VP was used simultaneously.Phi significantly inhibited tumor growth in nude mice.Compared with the Control group,the tumor volume,mass and Ki-67 positive rate of nude mice in the Phi group decreased significantly(all P<0.05),while the levels of LATS1 and p-YAP/YAP increased significantly(all P<0.05).Conclusion:Phi may inhibit the malignant biological behaviors of colon cancer LS180 cells by activating the Hippo/YAP signaling pathway.关键词
连翘苷/Hippo/Yes信号通路/结直肠癌/LS180细胞/增殖/迁移/侵袭/小鼠模型Key words
phillyrin(Phi)/Hippo/YAP signaling pathway/colorectal cancer(CRC)/LS180 cell/proliferation/migration/invasion/mouse model分类
医药卫生引用本文复制引用
郑成富,周贵丰,李青,陈营..连翘苷通过激活Hippo-YAP信号通路抑制结肠癌LS180细胞的恶性生物学行为[J].中国肿瘤生物治疗杂志,2024,31(6):566-572,7.基金项目
济宁医学院教师科研扶持基金(No.JYFC2018FKJ078) （No.JYFC2018FKJ078）
