神经药理学报2024，Vol.14Issue(2)：1-6,6.DOI:10.3969/j.issn.2095-1396.2024.02.001
醒脑静基于TLR4/NF-κB信号通路改善热射病大鼠神经炎症与认知功能障碍
Xingnaojing Improves Neuroinflammation and Cognitive Dysfunction in Heatstroke-Induced Rats Based on TLR4/NF-κB Signaling Pathway
摘要
Abstract
Objective:To observe the effect of Xingnaojing on inflammatory cytokines in brain tissues in heatstroke-induced rats,and to explore the mechanism of cognitive improvement.Methods:Thirty-six Wistar rats were randomly divided into control group,model group,and Xingnaojing group.The rats were intervened with high temperature of(41±0.5)℃ and high humidity of(75％±5％).The core temperature of the rats was monitored,when the temperature reach to 42℃,the rats were treated with cooling administration,and the Xingnaojing group were administrated with drugs i.v.After 3 days of continuous intervention,water maze tests were performed,and then brains were taken for the expression of inflammatory cytokines and related proteins dectection.Results:The results of the study showed that Xingnaojing had a significant protective effect on cognitive dysfunction in heatstroke-induced rats.The intervention of Xingnaojing significantly reduced the expression of inflammatory cytokines in the rat brain,inhibited the activation of microglia in the brain,and improved neuronal apoptosis in the hippocampal CA1 area.In addition,Xingnaojing significantly reversed the increased expression of TLR4 protein,increased NF-κB phosphorylation and increased NLRP3 protein expression in the rat brain caused by heatstroke.Conclusion:Xingnaojing can improve cognitive dysfunction by suppressing the inflammatory response in the brain of heatstroke disease.关键词
热射病/炎症反应/认知功能/TLR4/NF-κBKey words
heatstroke disease/inflammation/cognitive function/TLR4/NF-κB分类
医药卫生引用本文复制引用
郑丽丽,刘美岑..醒脑静基于TLR4/NF-κB信号通路改善热射病大鼠神经炎症与认知功能障碍[J].神经药理学报,2024,14(2):1-6,6.基金项目
福建省教育厅中青年科技类科研项目(No.JAT210905) （No.JAT210905）
