神经药理学报2024，Vol.14Issue(2)：7-13,7.DOI:10.3969/j.issn.2095-1396.2024.02.002
交泰丸含药血清通过激活SIRT1抑制内质网应激改善高糖高脂对SH-SY5Y细胞的损伤
Jiaotai Pill Drug-Containing Serum Ameliorates the Injury of SH-SY5Y Cells Induced by High Glucose/Palmitic Acid by Activating SIRT1 and Inhibiting Endoplasmic Reticulum Stress
摘要
Abstract
Objective:To investigate the effect of Jiaotai Pill on SH-SY5Y cell injury induced by high glucose and high lipid.Methods:Twenty Sprague Dawley rats were randomly divided into control group and Jiaotai Pill group,with 10 rats in each group.The rats in the Jiaotai Pill group were given Jiaotai Pill solution 8.4 g·kg-1 by gavage,and the rats in the control group were given normal saline by gavage for 7 consecutive days to prepare blank serum and Jiaotai Pill serum.SH-SY5Y cells were cultured in vitro and treated with different proportions of serum for 24 hours.CCK-8 assay was used to detect the effect of serum containing Jiaotai Pill on the proliferation of SH-SY5Y cells.The cells were and divided into control group,low dose group and high dose group,SIRT1 inhibitor EX527 group according to the proportion of drug-containing serum and cultured with 25 mmol·L-1 high glucose and 200 μmol·L-1 palmitic acid or normal medium.Endoplasmic reticulum(ER)associated proteins[inositol-requiring enzyme 1α(IRE1α),phospho-inositol-requiring enzyme 1α(p-IRE1α),protein kinase R-like endoplasmic reticulum kinase(PERK),phospho-protein kinase R-like endoplasmic reticulum kinase(p-PERK),activating transcription factor 6(ATF6)and glucose regulated protein 78(GRP78)、protein disulfide isomerase(PDI)]were detected by Western blot.Immunofluorescence was used to detect the expression of energy metabolism-related proteins(SIRT1、p-IRE1α、p-PERK)in each group.Results:Compared with the control group,GRP78,the ratio of p-PERK to PERK and the ratio of p-IRE1α to IRE1α were significantly increased in the model group(P<0.05),SIRT1 and PDI were significantly decreased(P<0.05).Compared with the model group,the expressions of SIRT1 and PDI were up-regulated(P<0.05),and the expressions of GRP78,p-PERK/PERK,and p-IRE1α/IRE1α were down-regulated(P<0.05)in a concentration-dependent way in Jiaotai Pill low and high concentration groups.EX527 group significantly reversed the effect of Jiaotai pill(P<0.05).Conclusion:The drug-containing serum of Jiaotai Pill improve the high glucose/palmitic acid induced injury of SH-SY5Y cells by improving SIRT1 and inhibiting endoplasmic reticulum stress.关键词
交泰丸/高糖高脂/SIRT1/内质网应激/未折叠蛋白反应Key words
Jiaotai Pill/high glucose/palmitic acid/SIRT1/endoplasmic reticulum stress/unfolded protein response分类
医药卫生引用本文复制引用
黎沛森..交泰丸含药血清通过激活SIRT1抑制内质网应激改善高糖高脂对SH-SY5Y细胞的损伤[J].神经药理学报,2024,14(2):7-13,7.基金项目
国家自然科学基金项目(No.82004246) （No.82004246）
