组织工程与重建外科杂志2024，Vol.20Issue(3)：285-292,8.DOI:10.3969/j.issn.1673-0364.2024.03.002
内源性骨膜引导骨再生过程中信号通路富集及FZD家族动态表达研究
Enrichment of signaling pathways and dynamic expression of the FZD family during endogenous periosteum-guided bone regeneration
摘要
Abstract
Objective To investigate the signaling pathway enrichment and dynamic expression of FZD family during endogenous periosteum-guided bone regeneration.Methods A porcine closed rib periosteum-guided bone regeneration model was established,and periosteum and regenerated bone tissues were collected at seven time points for gene sequencing,differential gene screening and enrichment analysis of their related signaling pathways.The FZD molecular family,which is closely related to the process of bone regeneration,was selected for focused analysis,and the dynamic expression changes of each member of FZD and the expression correlation between them were detected and analyzed.Results A total of seven genes were persistently differentially expressed at different time points within 1 month after surgery in the experimental group compared with the control group.Differential gene enrichment showed that there was mainly an immune response in the early stage,and tissue development and remodeling were achieved later.The expression of each member of the FZD family fluctuated at different time points,and the expression peak of most members was 3 days after surgery,while the fold change of the experimental group was more obvious than that of the control group at 1 month after surgery,and FZD1 and FZD2 were the highest,and there was a high correlation between them.Conclusion The differential genes in the process of periosteum-guided bone regeneration and the related signaling pathways screened in this study provide a theoretical basis for further molecular mechanism studies,while the dynamic expression changes of the FZD molecular family play an important role in the process,and the specific biological mechanisms involved in the process need to be verified by further studies.关键词
大段骨缺损/骨膜/FZD家族/内源性引导骨再生Key words
Large segmental bone defects/Periosteum/FZD family/Endogenous guided bone regeneration分类
医药卫生引用本文复制引用
陈晓雪,余宝富,王紫,吴其峰,李青峰,魏皎..内源性骨膜引导骨再生过程中信号通路富集及FZD家族动态表达研究[J].组织工程与重建外科杂志,2024,20(3):285-292,8.基金项目
国家自然科学基金(81871572,82302820,82372546) （81871572,82302820,82372546）
上海市临床重点专科(shslczdzk00901) （shslczdzk00901）
上海市科学技术委员会临床医学研究中心项目(22MC1940300). （22MC1940300）
