中国实验动物学报2024，Vol.32Issue(6)：712-720,9.DOI:10.3969/j.issn.1005-4847.2024.06.004
围绝经期综合征肾阴虚证大鼠汗液分泌及其生化指标的变化
Sweat secretion and biochemical indexes in Yin-deficient ovariectomized rats
摘要
Abstract
Objective To assess the sweat secretion of Yin-deficient ovariectomized rats and investigate the changes in biochemical indexes.Methods Eighteen SD female rats were randomly divided into a sham operation group,model group,and positive control group of six rats each.The rats in the sham operation group underwent a sham operation,and those in the model group and positive control group underwent bilateral ovariectomy.L-Thyroxine(92 mg/kg)was given once a day for 7 consecutive days starting on the 7th postoperative day to establish a Yin-deficient ovariectomized model.The rats in the positive control group were orally administered Qinggu San Tang(7.3 g/kg)once a day,while those in the sham operation group and model group were orally administered an equal amount of distilled water once a day for a total of 14 days.Sweat secretion from the plantar region of the foot was measured using the Wada-Takagaki reagent coloring method.At the end of the experiment,blood was taken from the abdominal aorta and the tissue of the paw pads was separated.The serum levels cyclic adenosine monophosphate(cAMP),cyclic guanosine monophosphate(cGMP),luteinizing hormone(LH),gonadotropin-releasing hormone(GnRH),and estradiol(E2)were measured by enzyme-linked immunosorbent assay.Western Blot was used to determine the expression levels of M3 R,β2 AR,and aquaporin-5(AQP5)in the paw pad.Results The three main findings of this study were as follows.(1)Compared with the rats in the sham operation group,those in the model group were more irritable and aggressive,and their body weights decreased while their average temperature and sweat secretion significantly increased.(2)Serum cAMP level and cAMP/cGMP ratio increased,the LH and GnRH levels significantly increased,and the E2 level decreased.(3)M3 R expression was down-regulated and β2 AR and AQP5 expression was up-regulated in the paw pads of the rats.After 2 weeks of positive control treatment,the serum cAMP level and cAMP/cGMP ratio significantly decreased and the LH and GnRH levels decreased;however,no statistically significant difference was observed in the serum E2 level.The expression levels of M3 R were increased-regulated in paw pads of the rats,and reduced expression of β2 AR and AQP5.Conclusions Sweat secretion significantly increased in this"combined disease and evidence"model of perimenopausal syndrome kidney yin deficiency established by desiccation combined with thyroxine.The underlying mechanism may be related to the changes in cGMP,cAMP,and key proteins M3 R,β2 AR,and AQP5 in sweat glands that regulate sweat secretion.关键词
去势/肾阴虚证/汗液分泌/生化指标Key words
castration/syndrome of deficiency of kidney yin/sweat secretion/biochemical indexes分类
生物科学引用本文复制引用
王婕,陈雯佳,林文武,王颖峥,王英豪,黄美霞..围绝经期综合征肾阴虚证大鼠汗液分泌及其生化指标的变化[J].中国实验动物学报,2024,32(6):712-720,9.基金项目
国家中医药管理局临床中药学高水平中医药重点学科建设项目(国中医药人教函[2022]226 号),福建省自然科学基金资助项目(2023J01857),福建中医药大学基础类学科科研提升项目(XJC2022013).Funded by High-Level Key Discipline Construction Project of Clinical Traditional Chinese Medicine of the State Administration of Traditional Chinese Medicine(Guo Zhong Yao Ren Jiao Han[2022]No.226),Fujian Natural Science Foundation Funded Project(2023J01857),Fujian University of Traditional Chinese Medicine Basic Discipline Research Enhancement Project(XJC2022013). （国中医药人教函[2022]226 号）
