中国实验动物学报2024，Vol.32Issue(6)：753-761,9.DOI:10.3969/j.issn.1005-4847.2024.06.008
基于非稳态负荷评估雄性SD大鼠衰老进展及左归丸的干预作用
Using allostatic load as a tool for evaluating aging of male SD rats and the intervention effects of Zuogui Pill
摘要
Abstract
Objective Using the concept of allostatic load(AL)to evaluate aging of male SD rats and the effectiveness of Zuogui Pill in naturally aging rats.Methods Naturally aging male SD rats were tested at the ages of 2,5,8,14,18,and 21 months.They were divided into an elderly control group,low-dose Zuogui Pill group,and high-dose Zuogui Pill group.Intervention with Zuogui Pill was trialed for 3 months.Blood samples were taken from the tails of rats each month,and the number of T lymphocytes and rate of apoptosis were measured by flow cytometry.Low-density lipoprotein cholesterol(LDL-c),high-density lipoprotein cholesterol(HDL-c),triglycerides(TG),total cholesterol(TC),free fatty acids(FFA),25 hydroxyvitamin D(25-OH-D),corticosterone(CORT),C-reactive protein(CRP),and interleukin-6(IL-6)were detected in rat sera.By identifying the collinearity between indicators and professional considerations,LDL-c,TC,HDL-c,FFA,TG,CORT,IL-6,CRP,25-OH-D,CD3+T cell count,and CD3+T cell apoptosis rate were included in the AL scoring.The threshold for each indicator was established with data from 5-month-old rats,and the score was 1 point below or/and above the threshold.Results The serum levels of LDL-c,TG,TC,25-OH-D,CRP,and IL-6 of rats showed significant changes with age,although the patterns of change differed.The CD3+T lymphocyte count significantly decreased with age(P＜0.01),while the apoptosis rates of CD3+,CD4+,and CD8+T lymphocytes significantly increased with age(P＜0.01).Zuogui Pill significantly increased serum CORT levels in elderly rats(P＜0.01)and reduced the apoptosis rate of CD8+T lymphocytes(P＜0.05).The AL score began to increase in rats at 5 months of age and reached its peak in those of 18 months of age.Conclusions AL can better characterize the aging process compared to a single indicator.Zuogui Pill can improve the stress response ability of aging rats and alleviate immunosenescence.关键词
非稳态负荷/衰老/左归丸/免疫衰老Key words
allostatic load/aging/Zuogui Pill/immunosenescence分类
生物科学引用本文复制引用
贾雨欣,邓晓红,孙磊峰,陈丹丹,孙贤俊,段晓红,严诗楷,黄建华..基于非稳态负荷评估雄性SD大鼠衰老进展及左归丸的干预作用[J].中国实验动物学报,2024,32(6):753-761,9.基金项目
上海市扬帆计划(21YF1404000).Funded by Shanghai Sailing Program(21YF1404000). （21YF1404000）
