青钱柳降糖降尿酸活性部位色谱指纹图谱研究及主成分含量测定OA北大核心CSTPCD

This paper carried out the content determination and fingerprinting study of the hypoglyce-mic and uric acid-lowering active parts of Cyclocarya paliurus.(C.paliurus.),and evaluated the quality of C.paliurus.herbs from different origins.Under the guidance of activity screening,the ex-traction conditions were optimized.Using 30％ethanol aqueous solution as extraction solvent,a sol-id-liquid ratio of 1∶10,and ultrasonic extraction for 1 hour can extract the active part effectively.A high performance liquid chromatography(HPLC)method was used for the determination of the con-tents of chlorogenic acid,quercetin-3-O-β-D-glucuronide,quercitrin,kaempferol-3-O-β-D-glucuro-nide,isochlorogenic acid C and afzelin in the active part of C.paliurus..Quality evaluation was based on the content variation of each ingredients with the origin.Among them,the Jiangxi herb S1 had the low content,which differed from the herbs of other origins.At the same time,a fingerprint of the active part of C.paliurus.was established.The similarity of the 10 batches of herbs was all greater than 0.9.PCA analysis showed that the quality differences of Jiangxi herb S1,Hunan herbs S6 and S8 were large.The inhibitory activities of α-glucosidase and xanthine oxidase showed that Hu-nan herb S5 had the best hypoglycemic and uric acid-lowering activities,while Jiangxi herb S1 per-formed poorly,providing consistent conclusions with quality evaluation.The established method pro-vide a scientific basis for the quality control and the quality standard of C.paliurus.herb.